Human Lung Squamous Cell Carcinoma Organoid Kit 

MCE Human Lung Squamous Cell Carcinoma Organoid Kit contains Lung Squamous Cell Carcinoma Organoid Basal Medium A, Lung Squamous Cell Carcinoma Organoid Supplement B (50×), Lung Squamous Cell Carcinoma Organoid Supplement C (250×). This product can be used to efficiently construct human Lung Squamous Cell Carcinoma organoid.

The organoid of lung squamous cell carcinoma can highly simulate the tumor microenvironment of lung squamous cell carcinoma, which facilitates the observation of the growth and proliferation of lung squamous cell carcinoma cells and can be used to track the changes of tumor cells under the action of drugs and screen the efficacy and toxicity of drugs.

Basal Medium A : 4℃, 1 year. Shipping with blue ice.

Supplement B (50×) & Supplement C (250×) : -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry ice.

1. Tumor cells need to be kept sterile when extracted from primary tumor tissue to avoid contamination from subsequent experiments.

2. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

3. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.

1. Tumor cells need to be kept sterile when extracted from primary tumor tissue to avoid contamination from subsequent experiments.

2. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

3. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.