1. MCE Kits
  2. Protein Biology
  3. Protein Purification
  4. Magnetic Beads
  5. Anti-MBP Magnetic Beads

Anti-MBP Magnetic Beads 

Cat. No.: HY-K0250
Manual COA SDS Technical Support

Anti-MBP Magnetic Beads are suitable for the detection and purification of MBP-tagged fusion proteins, as well as for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) applications.

Anti-MBP Magnetic Beads
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  • Description

  • Storage

  • Attention

  • Components

  • Documentation

Description
& Advantages

Maltose Binding Protein (MBP) is a maltose/maltodextrin-binding protein derived from Escherichia coli, with a molecular weight of approximately 42 kDa.

The MBP tag offers the following advantages: it significantly enhances the solubility of heterologous proteins, particularly those expressed as inclusion bodies; it can be expressed in Escherichia coli or yeast systems to improve the expression level of target proteins; it promotes proper protein folding; the MBP tag can be removed by specific proteases such as PreScission, TEV, or Thrombin; it enables a simple and highly specific purification process. Additionally, the MBP tag is suitable for studies involving protein expression, localization, purification, and immunoassays. Due to these advantages, the MBP tag is widely used in protein research.

MCE Anti-MBP Magnetic Beads are prepared by covalently coupling high-quality mouse monoclonal anti-MBP antibodies to magnetic beads. These beads offer high binding capacity, excellent specificity, and stable performance. Anti-MBP Magnetic Beads are suitable for the detection and purification of MBP-tagged fusion proteins, as well as for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) applications.

Storage

4°C, 2 years

Do not dry or freeze

Attention

1. Do not centrifuge, dry or freeze the magnetic beads, which will cause the beads to aggregate and lose binding affinity.

2. To minimize protein degradation, protease inhibitor cocktails (MCE Cat. No. HY-K0010, HY-K0011) are highly recommended.

3. Do not use cell lysates containing dithiothreitol (DTT), as DTT may cause the antibody to detach from the magnetic beads.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.

Components
Components HY-K0250-1 mL HY-K0250-5 mL
Anti-MBP Magnetic Beads 1 mL 1 mL × 5
Documentation

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Anti-MBP Magnetic Beads
Cat. No.:
HY-K0250
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