INF7 

INF7 is a derivative of the N-terminal domain of the HA2 protein and is sensitive to pH. INF7 disrupts the stability of endosomal membranes through a mechanism independent of membrane fusion. INF7 can be used to enhance the endosome escape of complex or liposome-encapsulated proteins. Co-encapsulation of INF7 and molecular imaging probes in liposomes can enhance intracellular signaling and probe retention^[1][2].

Post-modification of the R8-MEND with INF7 results in a 4-fold increased gene expression, aggregates of R8-MEND particles showing neutral to negative ζ-potential^[1].
After endocytosis by CV1 cells, the INF7 (500 μg/mL) peptide is activated by the acidic pH value of the endosome and promotes the effective release of the fluorescent tracer into the cytosol^[2].
INF7 500 mu (g/mL) to promote significantly enhanced intracellular escape not the initiative from the eduction in cytoplasm sol of tracer retention^[2].

2693.96

C₁₂₄H₁₆₉N₂₇O₃₇S₂

185462-59-3

Solid

White to off-white

Gly-Leu-Phe-Glu-Ala-Ile-Glu-Gly-Phe-Ile-Glu-Asn-Gly-Trp-Glu-Gly-Met-Ile-Asp-Gly-Trp-Tyr-Gly-Cys

GLFEAIEGFIENGWEGMIDGWYGC

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Ayman El-Sayed, et al. Enhanced gene expression by a novel stearylated INF7 peptide derivative through fusion independent endosomal escape. J Control Release. 2009 Sep 1;138(2):160-7.  [Content Brief]

  [2]. Burks SR, et al. Co-encapsulating the fusogenic peptide INF7 and molecular imaging probes in liposomes increases intracellular signal and probe retention. PLoS One. 2015 Mar 27;10(3):e0120982.  [Content Brief]