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  3. INDO 1 pentapotassium

INDO 1 pentapotassium is a fluorescent Ca2+ indicator, binds specifically to Ca2+ while emitting fluorescence, the maximum emission wavelength shifts from 485 nm before binding to 410 nm.

For research use only. We do not sell to patients.

INDO 1 pentapotassium Chemical Structure

INDO 1 pentapotassium Chemical Structure

CAS No. : 132319-56-3

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Based on 1 publication(s) in Google Scholar

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Description

INDO 1 pentapotassium is a fluorescent Ca2+ indicator, binds specifically to Ca2+ while emitting fluorescence, the maximum emission wavelength shifts from 485 nm before binding to 410 nm[1].

Molecular Weight

840.05

Formula

C32H26K5N3O12

CAS No.
Appearance

Solid

Color

Off-white to light yellow

Emission (Em)

475

Excitation (Ex)

350

SMILES

O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(O[K])=O)CC(O[K])=O)C(OCCOC4=CC(C)=CC=C4N(CC(O[K])=O)CC(O[K])=O)=C3)N2)O[K]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (74.40 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1904 mL 5.9520 mL 11.9041 mL
5 mM 0.2381 mL 1.1904 mL 2.3808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1904 mL 5.9520 mL 11.9041 mL 29.7601 mL
5 mM 0.2381 mL 1.1904 mL 2.3808 mL 5.9520 mL
10 mM 0.1190 mL 0.5952 mL 1.1904 mL 2.9760 mL
15 mM 0.0794 mL 0.3968 mL 0.7936 mL 1.9840 mL
20 mM 0.0595 mL 0.2976 mL 0.5952 mL 1.4880 mL
25 mM 0.0476 mL 0.2381 mL 0.4762 mL 1.1904 mL
30 mM 0.0397 mL 0.1984 mL 0.3968 mL 0.9920 mL
40 mM 0.0298 mL 0.1488 mL 0.2976 mL 0.7440 mL
50 mM 0.0238 mL 0.1190 mL 0.2381 mL 0.5952 mL
60 mM 0.0198 mL 0.0992 mL 0.1984 mL 0.4960 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
INDO 1 pentapotassium
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HY-D0121A
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