ICRF-193 

ICRF-193 is a DNA Topoisomerase II inhibitor. (S,S)- and (R,R)-isomers ICRF-193 make up an racemic mixture, ICRF-196 (HY-118590A). ICRF-193 can inhibit DNA syntheses and induces apoptosis. ICRF-193 exhibits anti-cancer and anti-inflammation effects. ICRF-193 shows cardioprotective effect against anthracycline toxicity to cardiomyocytes. ICRF-193 can be used for the researches of cancer, infection, inflammation and cardiovascular disease, such as acute promyelocytic leukemia^{[1][2][3][4][5][6]}.

ICRF-193 (10-100 μM, 2 h) induces mitotic defects in fission yeast cells expressing histone H2A-RFP, α-tubulin Atb2-GFP and spindle pole body protein Sid4-GFP^[1].
ICRF-193 (100 μM. 2 h) inhibits its own induced snapped spindles and induces unequal chromosome segregation and unequal chromosome segregation in fission yeast cells^[1].
ICRF-193 (100 μM, 4 h) alters nuclear morphology and DNA content in cdc11-123 fission yeast mutant^[1].
ICRF-193 (100 μM, 8 h) reduces cell viability in wild-type fission yeast^[1].
ICRF-193 (5 days) inhibits the growth of human acute promyelocytic leukemia (APL) cell lines (NB4, HT-93) and other myeloid leukemia cell lines (HL-60, U937) with IC₅₀ of 0.21-0.26 μM^[2].
ICRF-193 (0.1-0.2 μM, 5 days) induces granulocytic differentiation of NB4, HT-93, HL-60, and U937 cells^[2].
ICRF-193 (0.2 μM, 48 h) significantly downregulates the level of PML-RAR fusion protein and upregulates p21 and RARβ levels in NB4 cells^[2].
ICRF-193 (10 μM) inhibits cells arrested at the quiescent (G0) phase reentry into the S phase and inhibits DNA syntheses in murine spleen cells^[3].
ICRF-193 (150 nM, 72 h) inhibits LPS (HY-D1056)-induced IL-1β secretion in human macrophage^[4].
ICRF-193 decreases DNA fragmentation induced by Etoposide (HY-13629) and induces apoptosis in murine thymocytes^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

282.30

C₁₂H₁₈N₄O₄

21416-88-6

Solid

White to off-white

C[C@@H]([C@@H](C)N(CC(N1)=O)CC1=O)N(CC(N2)=O)CC2=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Nakazawa N, et al. ICRF-193, an anticancer topoisomerase II inhibitor, induces arched telophase spindles that snap, leading to a ploidy increase in fission yeast. Genes Cells. 2016 Sep;21(9):978-93.  [Content Brief]

  [2]. Niitsu N, et al. The catalytic DNA topoisomerase II inhibitor ICRF-193 and all-trans retinoic acid cooperatively induce granulocytic differentiation of acute promyelocytic leukemia cells: candidate drugs for chemo-differentiation therapy against acute promyelocytic leukemia. Exp Hematol. 2002 Nov;30(11):1273-82.  [Content Brief]

  [3]. Hossain MS, et al. ICRF-193, a catalytic inhibitor of DNA topoisomerase II, inhibits re-entry into the cell division cycle from quiescent state in mammalian cells. Genes Cells. 2002 Mar;7(3):285-94  [Content Brief]

  [4]. Brindle A, et al. The Bisdioxopiperazine ICRF-193 Attenuates LPS-induced IL-1β Secretion by Macrophages. Inflammation. 2024 Feb;47(1):84-98.  [Content Brief]

  [5]. Jirkovská A, et al. Structure-Activity Relationship Study of Dexrazoxane Analogues Reveals ICRF-193 as the Most Potent Bisdioxopiperazine against Anthracycline Toxicity to Cardiomyocytes Due to Its Strong Topoisomerase IIβ Interactions. J Med Chem. 2021 Apr 8;64(7):3997-4019.  [Content Brief]

  [6]. Tanimoto C, et al. ICRF-193 modifies etoposide-induced apoptosis in thymocytes. Acta Med Okayama. 1995 Dec;49(6):281-6.  [Content Brief]