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  4. Hydroxy naphthol blue disodium

Hydroxy naphthol blue disodium, an azo dye, is a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions ( λmax = 650 nm). In the pH range between 12 and 13, the solution of the indicator is reddish pink in the presence of calcium ion and to deep blue in the presence of disodium EDTA.

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Hydroxy naphthol blue disodium Chemical Structure

Hydroxy naphthol blue disodium Chemical Structure

CAS No. : 165660-27-5

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Description

Hydroxy naphthol blue disodium, an azo dye, is a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions ( λmax = 650 nm). In the pH range between 12 and 13, the solution of the indicator is reddish pink in the presence of calcium ion and to deep blue in the presence of disodium EDTA[1].

Molecular Weight

598.49

Formula

C20H12N2Na2O11S3
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=C1C=C(S(=O)(O)=O)C2=CC=CC=C2/C1=N/NC3=C(O)C(S(=O)(O[Na])=O)=CC4=CC(S(=O)(O[Na])=O)=CC=C43
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (41.77 mM; Need ultrasonic)

DMSO : 5.83 mg/mL (9.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6709 mL 8.3544 mL 16.7087 mL
5 mM 0.3342 mL 1.6709 mL 3.3417 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.6709 mL 8.3544 mL 16.7087 mL 41.7718 mL
5 mM 0.3342 mL 1.6709 mL 3.3417 mL 8.3544 mL
H2O 10 mM 0.1671 mL 0.8354 mL 1.6709 mL 4.1772 mL
15 mM 0.1114 mL 0.5570 mL 1.1139 mL 2.7848 mL
20 mM 0.0835 mL 0.4177 mL 0.8354 mL 2.0886 mL
25 mM 0.0668 mL 0.3342 mL 0.6683 mL 1.6709 mL
30 mM 0.0557 mL 0.2785 mL 0.5570 mL 1.3924 mL
40 mM 0.0418 mL 0.2089 mL 0.4177 mL 1.0443 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Hydroxy naphthol blue disodium Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hydroxy naphthol blue disodium165660-27-5Fluorescent Dyecalcium indicatoralkaline earth metal ionsEDTAazo dyeInhibitorinhibitorinhibit

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