2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. Huib32

Huib32 is a potent small-molecule inhibitor of USP32 (IC50 = 21.2 nM), exhibiting high selectivity over other closely related deubiquitinating enzymes (DUBs), such as USP8/10/16, UCHL1 and OTUB2. Huib32 reversibly inhibits USP32 by covalently binding to the active site Cys743, which enhances substrate ubiquitination, alters endosomal morphology, and mimics USP32 depletion. Huib32 can be used for breast, ovarian, and lung cancer and Alzheimer's and Parkinson’s diseases research.

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Huib32

Huib32 Chemical Structure

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Huib32 Related Antibodies

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USP1 USP2 USP7 USP8 USP10 USP30 UCHL1

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Description

Huib32 is a potent small-molecule inhibitor of USP32 (IC50 = 21.2 nM), exhibiting high selectivity over other closely related deubiquitinating enzymes (DUBs), such as USP8/10/16, UCHL1 and OTUB2. Huib32 reversibly inhibits USP32 by covalently binding to the active site Cys743, which enhances substrate ubiquitination, alters endosomal morphology, and mimics USP32 depletion. Huib32 can be used for breast, ovarian, and lung cancer and Alzheimer's and Parkinson’s diseases research[1].

IC50 & Target[1]

USP32

21.2 nM (IC50)

In Vitro

Huib32 (BB01CA282) (0-50 μM, 6-24 h) selectively inhibits both USP32FL and USP32CD in a dose-dependent manner (from 0.1 μM), with no significant effect on 16 other detected DUBs[1].
Huib32 (0-50 μM, 24 h) demonstrates potent, dose-dependent target engagement of endogenous USP32 in MelJuSo cells (IC50 ~0.1 μM), confirming its cell permeability and cellular on-target activity[1].
Huib32 (10 μM, 4-72 h) induces late endosome dispersion and enhances ubiquitination of RAB7 and other substrates (e.g., RAB6, RAB32, RAB11A/B, TMEM192), confirming its role in membrane trafficking and revealing new potential substrates of USP32[1].
Huib32 (10 μM, 4-72 h) significantly alters the ubiquitinome, enriching and depleting unique Lys-ε-Gly-Gly peptides at 4 h and 72 h, impacting substrates like RAB11A/B and USP32 itself without changing their total protein levels, indicating non-proteolytic ubiquitination[1].
Huib32 (10 μM, 72 h) specifically increased the ubiquitination of GFP-RAB7 WT, but not the GFP-RAB7 2KR mutant, validating its on-target effect against an established USP32 substrate[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Huib32 Related Antibodies

Western Blot Analysis[1]

Cell Line: MelJuSo cells
Concentration: 0, 0.1, 0.5, 1, 5, 10, and 50 μM
Incubation Time: 4, 24 and 72 h
Result: Strongly increased the ubiquitination of RAB7 after 4 h of incubation in MelJuSo cells stably expressing GFP-RAB7, which steadily stayed up to 72 h of incubation.
Approximately 2-fold increase in RAB7 ubiquitination was observed in GFP-RAB7 WT, but not in the ubiquitination-deficient mutant GFP-RAB7 2KR.

Immunofluorescence[1]

Cell Line: MelJuSo cells
Concentration: 0, 5 and 10 μM
Incubation Time: 72 h
Result: Induced dispersion and swelling of the late endosomal compartments, a phenotype consistent with USP32 depletion.
Molecular Weight

381.45

Formula

C16H23N5O4S
SMILES

CC1=NOC(C)=C1S(N2CCC(C(N[C@@H]3CCN(C#N)C3)=O)CC2)(=O)=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Huib32 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Huib32Huib 32Huib-32DeubiquitinaseDUBsUSP32biquitination,DUBendosomalbreastovarianlung cancerAlzheimer'sParkinson’sInhibitorinhibitorinhibit

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