2. Protein Tyrosine Kinase/RTK
  3. FGFR
  4. HuGAL-FR21

HuGAL-FR21 is a humanized antiFGFR2IIIb IgG1 monoclonal antibody. HuGAL-FR21 can block the binding of FGF2, FGF7, and FGF10 to FGFR2IIIb and inhibit FGF induced phosphorylation of FGFR2IIIb. HuGAL-FR21 can downregulate the expression of FGFR2 in SNU-16 cells. HuGAL-FR21 shows the significant anti-tumor activity in athymic nude mice bearing gastric cancer xenograft models. HuGAL-FR21 can be used for research on cancer such as gastric cancer.

For research use only. We do not sell to patients.

HuGAL-FR21 Chemical Structure

HuGAL-FR21 Chemical Structure

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HuGAL-FR21 Related Antibodies

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Description

HuGAL-FR21 is a humanized antiFGFR2IIIb IgG1 monoclonal antibody. HuGAL-FR21 can block the binding of FGF2, FGF7, and FGF10 to FGFR2IIIb and inhibit FGF induced phosphorylation of FGFR2IIIb. HuGAL-FR21 can downregulate the expression of FGFR2 in SNU-16 cells. HuGAL-FR21 shows the significant anti-tumor activity in athymic nude mice bearing gastric cancer xenograft models. HuGAL-FR21 can be used for research on cancer such as gastric cancer[1].

IC50 & Target[1]

FGFR2

 

In Vitro

HuGAL-FR21 (0.001-1 μg/mL) can specifically bind to FGFR2IIIb (EC50 approximately 20 pM) and its mutant S252W in SNU-16 cells[1].
HuGAL-FR21 (0.01-100 μg/mL) effectively blocks the binding of FGFR2IIIb (IC50 approximately 1.5-3 μg/mL) to FGF2, FGF7, and FGF10[1].
HuGAL-FR21 (10 μg/mL, 30 min) effectively blocks FGF7 induced phosphorylation and significantly inhibits FGF2 induced phosphorylation in SNU-16 cells[1].
HuGAL-FR21 (10 μg/mL, 2-24 h) downregulates FGFR2 protein levels in SNU-16 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HuGAL-FR21 Related Antibodies

Western Blot Analysis[1]

Cell Line: SNU-16 cells
Concentration: 10 μg/mL
Incubation Time: 2, 8 and 48 h
Result: Reduced the level of FGFR2 protein by approximately 50%.
In Vivo

HuGAL-FR21 (0.5-5 mg/kg; i.p.; twice weekly; for 25-30 days) effectively inhibits the growth of tumors and has no obvious toxicity in athymic nude mice bearing SNU-16 xenografts or OCUM-2M xenografts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5×106 SNU-16 or OCUM-2M cells injected female athymic nude mice (5-6 weeks)[1]
Dosage: 0.5, 1, 2.5 and 5 mg/kg in SNU-16 model, 1 and 2.5 mg/kg in OCUM-2M model
Administration: Intraperitoneal injection (i.p.); twice weekly for 25 days in SNU-16 model and 30 days in OCUM-2M model
Result: Significantly inhibited the growth of SNU-16 and OCUM-2M tumors.
Did not show any other obvious toxicity.
Gene ID

2263  [NCBI]

Accession
Target

FGFR2/CD332
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

N/A
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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HuGAL-FR21 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HuGAL-FR21FGFRFibroblast growth factor receptorFGFR2FGF2FGF7SNU-16gastric cancerInhibitorinhibitorinhibit

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