HKSOX-1m (5/6-mixture) hydrobromide 

HKSOX-1m (5/6-mixture) hydrobromide is a O₂ fluorescent probe for mitochondria-targeting (Ex/Em=509/534nm; green), exhibiting excellent selectivity and sensitivity toward O₂ over a broad range of pH, strong oxidants, and abundant reductants found in cells^[1].

HKSOX-1m (5/6-mixture) hydrobromide (10 μM) sensitively captures signals for basal and Antimycin A (1 μM)-stimulated mitochondrial O₂^•− in differentiated human THP-1 cells, at very low laser power output^[1].

1. Preparation of HKSOX-1m (5/6-mixture) hydrobromide working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKSOX-1m (5/6-mixture) hydrobromide in 79 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKSOX-1m (5/6-mixture) hydrobromide working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKSOX-1m (5/6-mixture) hydrobromide working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1258.72

C₄₉H₃₇Br₂F₁₀N₂O₁₀PS₂

Solid

Light yellow to orange

534

509

O=C1OC(C(C2=C3F)=CC(F)=C3O[S](C(F)(F)F)(=O)=O)(C4=CC(C(N(CC5)CCN5CCCC[P+](C6=CC=CC=C6)(C7=CC=CC=C7)C8=CC=CC=C8)=O)=CC=C41)C(C(O2)=C9F)=CC(F)=C9O[S](C(F)(F)F)(=O)=O.O=C%10OC(C(C%11=C%12F)=CC(F)=C%12O[S](C(F)(F)F)(=O)=O)(C(C%10=C%13)=CC=C%13C(N(CC%14)CCN%14CCCC[P+](C%15=CC=CC=C%15)(C%16=CC=CC=C%16)C%17=CC=CC=C%17)=O)C(C(O%11)=C%18F)=CC(F)=C%18O[S](C(F)(F)F)(=O)=O.[Br-].Br.[Br-].Br

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Jun Jacob Hu, et al. Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo. J Am Chem Soc . 2015 Jun 3;137(21):6837-43.  [Content Brief]