2. Peptides
  3. Peptide and Derivatives
  4. Peptide Epitopes
  5. Tag Peptides
  6. HiBiT tag

HiBiT tag is a complimentary peptide (VSGWRLFKKIS), with high affinity for LgBiT. HiBiT tag is fused to the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT facilitates the stabilization of ETR and G protein. HiBiT is attached to a protein of interest (GFP) to quantify transport to the cytosol.

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HiBiT tag

HiBiT tag Chemical Structure

CAS No. : 1628912-54-8

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Based on 1 publication(s) in Google Scholar

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HiBiT tag Related Antibodies

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Description

HiBiT tag is a complimentary peptide (VSGWRLFKKIS), with high affinity for LgBiT. HiBiT tag is fused to the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT facilitates the stabilization of ETR and G protein. HiBiT is attached to a protein of interest (GFP) to quantify transport to the cytosol[1][2].

Molecular Weight

1320.58

Formula

C63H101N17O14
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Val-Ser-Gly-Trp-Arg-Leu-Phe-Lys-Lys-Ile-Ser
Sequence Shortening

VSGWRLFKKIS
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (75.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7572 mL 3.7862 mL 7.5724 mL
5 mM 0.1514 mL 0.7572 mL 1.5145 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7572 mL 3.7862 mL 7.5724 mL 18.9311 mL
5 mM 0.1514 mL 0.7572 mL 1.5145 mL 3.7862 mL
10 mM 0.0757 mL 0.3786 mL 0.7572 mL 1.8931 mL
15 mM 0.0505 mL 0.2524 mL 0.5048 mL 1.2621 mL
20 mM 0.0379 mL 0.1893 mL 0.3786 mL 0.9466 mL
25 mM 0.0303 mL 0.1514 mL 0.3029 mL 0.7572 mL
30 mM 0.0252 mL 0.1262 mL 0.2524 mL 0.6310 mL
40 mM 0.0189 mL 0.0947 mL 0.1893 mL 0.4733 mL
50 mM 0.0151 mL 0.0757 mL 0.1514 mL 0.3786 mL
60 mM 0.0126 mL 0.0631 mL 0.1262 mL 0.3155 mL
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HiBiT tag Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HiBiT tag1628912-54-8OthersInhibitorinhibitorinhibit

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