HGS101 

HGS101 is a fully human CCR5 monoclonal antibody with high affinity to CCR5. HGS101 binds to the 2nd extracellular loop (ECL-2) and acts as a signal antagonist. HGS101 restores Maraviroc (HY-13004) inhibition of Maraviroc-resistant HIV-1 infection of PBMCs. HGS101 shows anti-HIV activity by inhibiting CCR5 signaling in simian immunodeficiency virus-uninfected RMs models^[1][2].

CCR5/CD195

HGS101 (1 h) shows EC₅₀ values of 0.001 μg/mL for Maraviroc (HY-13004)-sensitive, 0.0003 μg/mL for Maraviroc-resistant, and 0.00002 μg/mL for Maraviroc-resistant HIV-1 in the presence of Maraviroc in JC6 cells^[1].

HGS101 (1 h) shows EC₅₀ values of 0.03 μg/mL for Maraviroc-sensitive, 0.03 μg/mL for Maraviroc-resresistant, and 0.01 μg/mL for Maraviroc-resistant HIV-1 in the presence of Maraviroc in PBMCs^[1].

HGS101 (0.3 µg/ mL, 1 h) restores Maraviroc inhibition (EC₅₀ of 0.007 nM) of Maraviroc-resistant HIV-1 in PBMCs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HGS101 (10 mg/kg, i.v., every 15 days for 10 times) shows anti-HIV activity by inhibiting CCR5 signaling in simian immunodeficiency virus-uninfected RMs models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1234  [NCBI]

P51681

CCR5/CD195

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

N/A

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Latinovic O, et al. CCR5 antibodies HGS004 and HGS101 preferentially inhibit drug-bound CCR5 infection and restore drug sensitivity of Maraviroc-resistant HIV-1 in primary cells. Virology. 2011 Mar 1;411(1):32-40.  [Content Brief]

  [2]. aaffe JE, et al. CCR5 blockade is well tolerated and induces changes in the tissue distribution of CCR5+ and CD25+ T cells in healthy, SIV-uninfected rhesus macaques. J Med Primatol. 2012 Feb;41(1):24-42.  [Content Brief]