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  3. Fluorescent Dye
  4. Halo tag TMR

Halo tag TMR is a red fluorescent dye composed of Halo tag ligand molecules and TMR. Halo tag can quickly and stably covalently bind to Halo protein with high specificity and affinity.

For research use only. We do not sell to patients.

Halo tag TMR Chemical Structure

Halo tag TMR Chemical Structure

CAS No. : 2764890-88-0

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Halo tag TMR Related Antibodies

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  • Biological Activity

  • Protocol

  • Purity & Documentation

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Description

Halo tag TMR is a red fluorescent dye composed of Halo tag ligand molecules and TMR. Halo tag can quickly and stably covalently bind to Halo protein with high specificity and affinity[1].

Molecular Weight

636.18

Formula

C35H42ClN3O6
CAS No.
Appearance

Solid

SMILES

C/[N+](C)=C1C=CC2=C(C3=CC(C(NCCOCCOCCCCCCCl)=O)=CC=C3C([O-])=O)C4=CC=C(N(C)C)C=C4OC2=C/1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (157.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5719 mL 7.8594 mL 15.7188 mL
5 mM 0.3144 mL 1.5719 mL 3.1438 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.36%

SDS (252 KB)

COA (245 KB) HNMR (232 KB) RP-HPLC (294 KB) LCMS (270 KB)

Handling Instructions (2659 KB)
References
Cell Assay

Guidelines (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
Staining Steps[1]:
1) Transfect cells with a plasmid encoding the HaloTag fusion protein of interest and grow to desired confluence under appropriate conditions.
2) Replace 1/5 of the culture medium with 5× HaloTag TMR working solution to a final concentration of 5 μM. Mix by gently shaking the culture vessel.
3) Incubate cells in a 37°C, CO2 incubator for 15 min to allow covalent binding of the ligand to the HaloTag protein.
4) For permeable ligands: Remove the medium containing the ligand and replace with an equal volume of pre-warmed fresh medium. Repeat this wash step two more times for a total of three washes. For cell surface markers (non-permeable ligands): Wash twice with fresh medium to remove excess ligand from the extracellular environment.
5) Continue incubating the cells in complete medium at 37°C for 30 min to ensure that unbound ligand is completely removed.
6) Replace the medium with phenol red-free medium (if available) to reduce autofluorescence.
7) Transfer the cells to a container suitable for imaging and observe using a fluorescence microscope.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5719 mL 7.8594 mL 15.7188 mL 39.2971 mL
5 mM 0.3144 mL 1.5719 mL 3.1438 mL 7.8594 mL
10 mM 0.1572 mL 0.7859 mL 1.5719 mL 3.9297 mL
15 mM 0.1048 mL 0.5240 mL 1.0479 mL 2.6198 mL
20 mM 0.0786 mL 0.3930 mL 0.7859 mL 1.9649 mL
25 mM 0.0629 mL 0.3144 mL 0.6288 mL 1.5719 mL
30 mM 0.0524 mL 0.2620 mL 0.5240 mL 1.3099 mL
40 mM 0.0393 mL 0.1965 mL 0.3930 mL 0.9824 mL
50 mM 0.0314 mL 0.1572 mL 0.3144 mL 0.7859 mL
60 mM 0.0262 mL 0.1310 mL 0.2620 mL 0.6550 mL
80 mM 0.0196 mL 0.0982 mL 0.1965 mL 0.4912 mL
100 mM 0.0157 mL 0.0786 mL 0.1572 mL 0.3930 mL
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Halo tag TMR Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Halo tag TMR2764890-88-0Fluorescent DyeHalo tagTMRchloroalkane-PEGInhibitorinhibitorinhibit

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