2. Metabolic Enzyme/Protease
  3. Acetyl-CoA Carboxylase
  4. hACC2-IN-1

hACC2-IN-1 is a potent acetyl-CoA carboxylase 2 (ACC2) inhibitor, with an IC50 value of 2.5 μM (hACC2). hACC2-IN-1 could be used for obesity research.

For research use only. We do not sell to patients.

hACC2-IN-1 Chemical Structure

hACC2-IN-1 Chemical Structure

CAS No. : 192323-14-1

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hACC2-IN-1 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

hACC2-IN-1 is a potent acetyl-CoA carboxylase 2 (ACC2) inhibitor, with an IC50 value of 2.5 μM (hACC2). hACC2-IN-1 could be used for obesity research[1].

IC50 & Target

hACC2

2.5 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
2.5 μM
Compound: 14
Inhibition of human recombinant his-tagged ACC2 expressed in baculovirus/Sf9 cell assessed as inorganic phosphate formation preincubated for 15 mins measured after 1.5 hrs by Malachite green reagent method
Inhibition of human recombinant his-tagged ACC2 expressed in baculovirus/Sf9 cell assessed as inorganic phosphate formation preincubated for 15 mins measured after 1.5 hrs by Malachite green reagent method
[PMID: 21515056]
In Vitro

ACC2 isoform, presents on the mitochondrial surface, inhibits carnitine palmitoyl transferase 1 (CPT-1) and converts acetyl-CoA into malonylCoA for de novo lipogenesis in the cytosol[1].
ACCs inhibition will change cellular substrate and favour lipid oxidation, thus preventing deleterious lipids from accumulating in oxidative tissue such as muscle, heart and liver[1].
hACC2-IN-1 shows good potency against the human ACC2 (hACC2) enzyme[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

hACC2-IN-1 Related Antibodies
Molecular Weight

432.58

Formula

C23H32N2O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C)(OC(NC[C@H]1CC[C@@H](CC1)CNS(=O)(C2=CC3=C(C=CC=C3)C=C2)=O)=O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (231.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3117 mL 11.5586 mL 23.1171 mL
5 mM 0.4623 mL 2.3117 mL 4.6234 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (11.56 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (11.56 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3117 mL 11.5586 mL 23.1171 mL 57.7928 mL
5 mM 0.4623 mL 2.3117 mL 4.6234 mL 11.5586 mL
10 mM 0.2312 mL 1.1559 mL 2.3117 mL 5.7793 mL
15 mM 0.1541 mL 0.7706 mL 1.5411 mL 3.8529 mL
20 mM 0.1156 mL 0.5779 mL 1.1559 mL 2.8896 mL
25 mM 0.0925 mL 0.4623 mL 0.9247 mL 2.3117 mL
30 mM 0.0771 mL 0.3853 mL 0.7706 mL 1.9264 mL
40 mM 0.0578 mL 0.2890 mL 0.5779 mL 1.4448 mL
50 mM 0.0462 mL 0.2312 mL 0.4623 mL 1.1559 mL
60 mM 0.0385 mL 0.1926 mL 0.3853 mL 0.9632 mL
80 mM 0.0289 mL 0.1445 mL 0.2890 mL 0.7224 mL
100 mM 0.0231 mL 0.1156 mL 0.2312 mL 0.5779 mL
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hACC2-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

hACC2-IN-1192323-14-1Acetyl-CoA CarboxylaseACC, Acetyl Coenzyme A Carboxylaseobese Zucker ratsacetyl-CoA carboxylasesACCsmalonyl-CoAligand lipophilicity efficiencyQuinolinehepatic malonyl-CoA levelInhibitorinhibitorinhibit

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