2. Anti-infection GPCR/G Protein
  3. HIV GPR39
  4. Glycolithocholic acid 3-sulfate disodium

Glycolithocholic acid 3-sulfate disodium  (Synonyms: Glycolithocholate sulfate disodium; Sulfolithocholylglycine disodium; SLCG disodium)

Cat. No.: HY-W587430 Purity: 99.73%
COA
SDS
Handling Instructions Technical Support

Glycolithocholic acid 3-sulfate disodium is a GPR39 agonist with EC50s of 47.9  and 66.8 μM (absence of Zn2+) and 8 and 8.7 μM (presence of Zn2+) in M39-20 and hGPR39-2 cells, respectively. Glycolithocholic acid 3-sulfate disodium stimulates GPR39 receptors to initiate intracellular calcium signaling, independent of Zn2+ binding sites H17 and H19. Glycolithocholic acid 3-sulfate disodium also inhibits replication of HIV-1 in vitro. Glycolithocholic acid 3-sulfate disodium can be used for the research of HIV infection and gallbladder disease.

For research use only. We do not sell to patients.

Glycolithocholic acid 3-sulfate disodium

Glycolithocholic acid 3-sulfate disodium Chemical Structure

CAS No. : 64936-82-9

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Based on 1 publication(s) in Google Scholar

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Description

Glycolithocholic acid 3-sulfate disodium is a GPR39 agonist with EC50s of 47.9  and 66.8 μM (absence of Zn2+) and 8 and 8.7 μM (presence of Zn2+) in M39-20 and hGPR39-2 cells, respectively. Glycolithocholic acid 3-sulfate disodium stimulates GPR39 receptors to initiate intracellular calcium signaling, independent of Zn2+ binding sites H17 and H19. Glycolithocholic acid 3-sulfate disodium also inhibits replication of HIV-1 in vitro. Glycolithocholic acid 3-sulfate disodium can be used for the research of HIV infection and gallbladder disease[1][2][3].

Molecular Weight

557.65

Formula

C26H41NNa2O7S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(NCC(O[Na])=O)=O)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4([C@](C[C@@H](CC4)OS(=O)(O[Na])=O)([H])CC3)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (17.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7932 mL 8.9662 mL 17.9324 mL
5 mM 0.3586 mL 1.7932 mL 3.5865 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (1.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (1.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.73%

SDS (393 KB)

COA (248 KB) HNMR (147 KB) RP-HPLC (244 KB) MS (152 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7932 mL 8.9662 mL 17.9324 mL 44.8310 mL
5 mM 0.3586 mL 1.7932 mL 3.5865 mL 8.9662 mL
10 mM 0.1793 mL 0.8966 mL 1.7932 mL 4.4831 mL
15 mM 0.1195 mL 0.5977 mL 1.1955 mL 2.9887 mL
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Glycolithocholic acid 3-sulfate disodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glycolithocholic acid 3-sulfate disodium64936-82-9Glycolithocholate sulfate disodiumSulfolithocholylglycine disodiumSLCG disodiumHIVGPR39Human immunodeficiency virusAgonistInhibitorHIV-1Zn2+M39-20 cellshGPR39-2 cellsgallbladder diseaseantiviralinhibitorinhibit

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