Glucagon (1-29), bovine, human hydrochloride (Synonyms: Glucagon hydrochloride)

Name: Glucagon (1-29), bovine, human hydrochloride
Brand: MedChemExpress
SKU: HY-P0082A
Rating: 5.0 (11 reviews)

Cat. No.: HY-P0082A Purity: 99.89%: Data Sheet
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Glucagon (1-29), bovine, human, porcine hydrochloride is a peptide hormone, produced by pancreatic α-cells. Glucagon hydrochloride stimulates gluconeogenesis. Glucagon (1-29), bovine, human, porcine hydrochloride activates HNF4α and increases HNF4α phosphorylation.

For research use only. We do not sell to patients.

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Glucagon (1-29), bovine, human hydrochloride Chemical Structure

CAS No. : 28270-04-4

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 11 publication(s) in Google Scholar

Other Forms of Glucagon (1-29), bovine, human hydrochloride:

Glucagon (1-29), bovine, human In-stock

11 Publications Citing Use of MCE Glucagon (1-29), bovine, human hydrochloride

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Description

Glucagon (1-29), bovine, human, porcine hydrochloride is a peptide hormone, produced by pancreatic α-cells. Glucagon hydrochloride stimulates gluconeogenesis^[1]. Glucagon (1-29), bovine, human, porcine hydrochloride activates HNF4α and increases HNF4α phosphorylation^[2]^[3].

In Vitro

Upon binding to its receptor Gcgr, Glucagon activates cAMP-PKA signaling to stimulate hepatic glucose production (HGP) and cause hyperglycemia^[1].
Glucagon stimulates both hepatic kisspeptin1 production and gluconeogenesis^[1].
Glucagon (100 nM) represses CYP7A1 mRNA expression in human primary hepatocytes^[3].
Glucagon (100 nM) increases phosphorylayion of HNF4α^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	Human primary hepatocytes (H1211, HH1215)
Concentration:	100 nM
Incubation Time:
Result:	Resulted in a marked increase in the amount of phosphorylated HNF4α.

In Vivo

Low-dose (20 μg/kg) Glucagon increases glycemia and does not stimulate insulin secretion in ambient-fed mice. High-dose (1 mg/kg) Glucagon lowers glycemia compared with PBS control and stimulates insulin secretion in ambient-fed mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J mice (12- to 24-week-old)^[4]
Dosage:	20 μg/kg and 1 mg/kg
Administration:	Administered by i.p. injection; 45 minutes
Result:	Low-dose (20 μg/kg) increased glycemia and did not stimulate insulin secretion. High-dose (1 mg/kg) lowered glycemia and stimulated insulin secretion.

Clinical Trial

Molecular Weight

36.46

Formula

ClH

CAS No.

28270-04-4

Appearance

Solid

Color

White to off-white

Sequence

His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr

Sequence Shortening

HSQGTFTSDYSKYLDSRRAQDFVQWLMNT

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (685.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 16.67 mg/mL (457.21 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	27.4273 mL	137.1366 mL	274.2732 mL
5 mM	5.4855 mL	27.4273 mL	54.8546 mL
10 mM	2.7427 mL	13.7137 mL	27.4273 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (284 KB) SDS (252 KB)

COA (242 KB) LCMS (100 KB) Elemental Analysis Report (102 KB)

Handling Instructions (2659 KB)

References

[1]. Song WJ, et al. Glucagon regulates hepatic kisspeptin to impair insulin secretion. Cell Metab. 2014 Apr 1;19(4):667-81. [Content Brief]

[2]. Hirota K, et al. Hepatocyte nuclear factor-4 is a novel downstream target of insulin via FKHR as a signal-regulated transcriptional inhibitor. J Biol Chem. 2003 Apr 11;278(15):13056-60. [Content Brief]

[3]. Song KH, et al. Glucagon and cAMP inhibit cholesterol 7alpha-hydroxylase (CYP7A1) gene expression in humanhepatocytes: discordant regulation of bile acid synthesis and gluconeogenesis. Hepatology. 2006 Jan;43(1):117-25. [Content Brief]

[4]. Capozzi ME, et al. Glucagon lowers glycemia when β-cells are active. JCI Insight. 2019 Jul 23;5. pii: 129954. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	27.4273 mL	137.1366 mL	274.2732 mL	685.6830 mL
	5 mM	5.4855 mL	27.4273 mL	54.8546 mL	137.1366 mL
	10 mM	2.7427 mL	13.7137 mL	27.4273 mL	68.5683 mL
	15 mM	1.8285 mL	9.1424 mL	18.2849 mL	45.7122 mL
	20 mM	1.3714 mL	6.8568 mL	13.7137 mL	34.2841 mL
	25 mM	1.0971 mL	5.4855 mL	10.9709 mL	27.4273 mL
	30 mM	0.9142 mL	4.5712 mL	9.1424 mL	22.8561 mL
	40 mM	0.6857 mL	3.4284 mL	6.8568 mL	17.1421 mL
	50 mM	0.5485 mL	2.7427 mL	5.4855 mL	13.7137 mL
	60 mM	0.4571 mL	2.2856 mL	4.5712 mL	11.4280 mL
	80 mM	0.3428 mL	1.7142 mL	3.4284 mL	8.5710 mL
	100 mM	0.2743 mL	1.3714 mL	2.7427 mL	6.8568 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Glucagon (1-29), bovine, human hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glucagon (1-29), bovine, human28270-04-4GlucagonGCGRGlucagon Receptorpeptidehormonepancreatic αgluconeogenesisHNF-4HNF4αInhibitorinhibitorinhibit

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Glucagon (1-29), bovine, human hydrochloride (Synonyms: Glucagon hydrochloride)

Customer Review

Other Forms of Glucagon (1-29), bovine, human hydrochloride:

Glucagon (1-29), bovine, human hydrochloride Related Antibodies

11 Publications Citing Use of MCE Glucagon (1-29), bovine, human hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Glucagon (1-29), bovine, human hydrochloride Related Antibodies

Molarity Calculator

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Complete Stock Solution Preparation Table

Glucagon (1-29), bovine, human hydrochloride Related Classifications

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Customer Review

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