2. Immunology/Inflammation NF-κB Apoptosis
  3. Toll-like Receptor (TLR) NF-κB Caspase TNF Receptor Interleukin Related
  4. GlcNAc-MurNAc

GlcNAc-MurNAc, a disaccharide, is a TLR4 agonist with a Kd of 383 μM for murine TLR4. GlcNAc-MurNAc directly binds to TLR4 and activates its downstream NF-κB and IRF pathways. GlcNAc-MurNAc improves Dextran sulfate sodium salt (DSS) (HY-116282C)-induced colitis in mice through a TLR4-dependent mechanism. GlcNAc-MurNAc can be used for the study of inflammatory bowel disease.

For research use only. We do not sell to patients.

GlcNAc-MurNAc Chemical Structure

GlcNAc-MurNAc Chemical Structure

CAS No. : 4500-70-3

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GlcNAc-MurNAc Related Antibodies

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Description

GlcNAc-MurNAc, a disaccharide, is a TLR4 agonist with a Kd of 383 μM for murine TLR4. GlcNAc-MurNAc directly binds to TLR4 and activates its downstream NF-κB and IRF pathways. GlcNAc-MurNAc improves Dextran sulfate sodium salt (DSS) (HY-116282C)-induced colitis in mice through a TLR4-dependent mechanism. GlcNAc-MurNAc can be used for the study of inflammatory bowel disease[1].

IC50 & Target[1]

TLR4

383 μM (Kd)

NF-κB

 

IL-6

 

IL-12

 

IL-1α

 

In Vitro

GlcNAc-MurNAc (GM) (1-8 mM, 24 h) is immunologically active via NOD-independent mechanisms in RAW264.7, BMDMs and THP-1 cells[1].
GlcNAc-MurNAc (4 mM, 24 h) depends on TLR4 to activate cellular immunity in WT bone-marrow-derived macrophages (BMDMs)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

GlcNAc-MurNAc Related Antibodies

ELISA Assay[1]

Cell Line: RAW264.7, BMDMs and THP-1 cells
Concentration: 1, 2, 4 mM for RAW264.7 cells; 2, 4, 8 mM for BMDMs and THP-1 cells
Incubation Time: 24 h
Result: Upregulated expression and production of proinflammatory cytokines TNF-α and IL-6.
Appeared less potent monosaccharide M.
Suppressed TNF-α production in RAW264.7 cells with the addition of TAK-242, a selective TLR4 inhibitor.

RT-PCR[1]

Cell Line: WT and Tlr4-/- BMDMs
Concentration: 4 mM
Incubation Time: 24 h
Result: Significantly upregulated some cytokine and chemokine genes, including IL12a/b, IL6, IL1a/b, Csf3, Ccl5, and Cxcl1 in WT cells but failed in Tlr4-/- cells.
In Vivo

GlcNAc-MurNAc (GM) (10 mg/kg, i.p., once daily for 9 days) mitigates DSS-induced colitis in mice via TLR4-dependent mechanisms[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS-induced colitis model established in 7-9 weeks WT and Tlr4-/- C57BL/6 J mice[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection (i.p.), once daily for 9 days (2 days before the DDS water administration)
Result: Alleviated colitis in WT mice, as evidenced by reduced body weight loss and colonic shortening in WT group.
Significantly reduced monocytes and neutrophils, in the colonic lamina propria in WT group.
Suppressed colonic expressions of proinflammatory genes, such as Tnfa, IL1b, and Ccl2 in WT group.
Restored the elevation of inflammatory markers, including IL-6, IFN-β, IL-27, IL-10, and IL-23 in WT group.
Failed to prevent weight loss and colonic shortening in Tlr4-/- group.
Molecular Weight

496.46

Formula

C19H32N2O13
CAS No.
SMILES

OC([C@@H](C)O[C@H]([C@H]([C@@H](O[C@@H]1CO)O)NC(C)=O)[C@@H]1O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO)O)O)NC(C)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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GlcNAc-MurNAc Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GlcNAc-MurNAc4500-70-3Toll-like Receptor (TLR)NF-κBCaspaseTNF ReceptorInterleukin RelatedNuclear factor-κBNuclear factor-kappaBTumor Necrosis Factor ReceptorTNFRILColitisGut microbiotaInflammatory bowel diseaseBone-marrow-derived macrophagesInhibitorinhibitorinhibit

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