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  3. Bacterial Parasite Antibiotic
  4. G-418

G-418  (Synonyms: Geneticin; Antibiotic G-418)

Cat. No.: HY-108718 Purity: 99.04%
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G-418 (Geneticin) is an aminoglycoside antibiotic with a structure similar to gentamicin. It is toxic to both eukaryotic and prokaryotic cells and works by interfering with protein synthesis.

For research use only. We do not sell to patients.

G-418

G-418 Chemical Structure

CAS No. : 49863-47-0

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 48 publication(s) in Google Scholar

Other Forms of G-418:

G-418 Related Antibodies

Top Publications Citing Use of Products

48 Publications Citing Use of MCE G-418

View All Parasite Isoform Specific Products:

View All Isoforms
Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

View All Antibiotic Isoform Specific Products:

View All Isoforms
Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

G-418 (Geneticin) is an aminoglycoside antibiotic with a structure similar to gentamicin. It is toxic to both eukaryotic and prokaryotic cells and works by interfering with protein synthesis[1].

IC50 & Target

Aminoglycoside

 

Cellular Effect
Cell Line Type Value Description References
Sf21 IC50
2 μM
Compound: G418
Inhibition of ribosomal activity in Spodoptera frugiperda Sf21 cells assessed as inhibition of translation after 60 mins by fluorescence assay
Inhibition of ribosomal activity in Spodoptera frugiperda Sf21 cells assessed as inhibition of translation after 60 mins by fluorescence assay
[PMID: 20409719]
In Vitro

G-418 (500 μg/mL, 12 days) was used to screen drug-resistant LNCaP cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

G-418 Related Antibodies
Molecular Weight

496.55

Formula

C20H40N4O10
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@@H]1O[C@@]([C@@H]([C@@H](O)[C@@H]2O)N)([H])O[C@]2([H])[C@H](O)C)[C@H]([C@@H](C[C@@H]1N)N)O[C@](OC[C@](C)(O)[C@@H]3NC)([H])[C@@H]3O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (251.74 mM; Need ultrasonic)

DMSO : 25 mg/mL (50.35 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0139 mL 10.0695 mL 20.1390 mL
5 mM 0.4028 mL 2.0139 mL 4.0278 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.0139 mL 10.0695 mL 20.1390 mL 50.3474 mL
5 mM 0.4028 mL 2.0139 mL 4.0278 mL 10.0695 mL
10 mM 0.2014 mL 1.0069 mL 2.0139 mL 5.0347 mL
15 mM 0.1343 mL 0.6713 mL 1.3426 mL 3.3565 mL
20 mM 0.1007 mL 0.5035 mL 1.0069 mL 2.5174 mL
25 mM 0.0806 mL 0.4028 mL 0.8056 mL 2.0139 mL
30 mM 0.0671 mL 0.3356 mL 0.6713 mL 1.6782 mL
40 mM 0.0503 mL 0.2517 mL 0.5035 mL 1.2587 mL
50 mM 0.0403 mL 0.2014 mL 0.4028 mL 1.0069 mL
H2O 60 mM 0.0336 mL 0.1678 mL 0.3356 mL 0.8391 mL
80 mM 0.0252 mL 0.1259 mL 0.2517 mL 0.6293 mL
100 mM 0.0201 mL 0.1007 mL 0.2014 mL 0.5035 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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G-418 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

G-41849863-47-0Geneticin Antibiotic G-418G418G 418Antibiotic G418Antibiotic G 418Antibiotic G-418BacterialParasiteAntibioticaminoglycosidegentamicineukaryoticprokaryoticInhibitorinhibitorinhibit

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