Fluoresterol  (Synonyms: 22-NBD Cholesterol)

Fluoresterol is a fluorescently labeled cholesterol analog that can be used as a fluorescent probe to study the behavior and metabolism of cholesterol in cells, including lipid uptake and esterification analysis, intracellular localization and targeting, and lipid metabolism in mammalian and bacterial cells. Fluoresterol has an excitation maximum at 472 nm and an emission maximum at 540 nm. Fluoresterol is expected to be useful in the study of metabolic diseases^[1][2].

494.63

C₂₈H₃₈N₄O₄

78949-95-8

Solid

Yellow to orange

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CNC3=CC=C([N+]([O-])=O)C4=NON=C34)([H])[C@@]5([H])[C@]([C@@]6(C(C[C@@H](O)CC6)=CC5)C)([H])CC1

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Faletrov Y V, et al. 22-NBD-cholesterol as a novel fluorescent substrate for cholesterol-converting oxidoreductases[J]. The Journal of steroid biochemistry and molecular biology, 2013, 134: 59-66.  [Content Brief]

  [2]. Lajdova I, et al. Spectrally and time-resolved fluorescence imaging of 22-NBD-cholesterol in human peripheral blood mononuclear cells in chronic kidney disease patients[J]. Molecules, 2021, 26(22): 6800.  [Content Brief]