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Fluorescent brightener 220 is an optical brightener dye.

For research use only. We do not sell to patients.

Fluorescent brightener 220

Fluorescent brightener 220 Chemical Structure

CAS No. : 16470-24-9

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Description

Fluorescent brightener 220 is an optical brightener dye.

Molecular Weight

1165.04

Formula

C40H40N12Na4O16S4

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC=C(NC2=NC(N(CCO)CCO)=NC(NC3=CC=C(C(S(=O)(O[Na])=O)=C3)/C=C/C4=CC=C(NC5=NC(NC6=CC=C(S(=O)(O[Na])=O)C=C6)=NC(N(CCO)CCO)=N5)C=C4S(=O)(O[Na])=O)=N2)C=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (28.61 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8583 mL 4.2917 mL 8.5834 mL
5 mM 0.1717 mL 0.8583 mL 1.7167 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.8583 mL 4.2917 mL 8.5834 mL 21.4585 mL
5 mM 0.1717 mL 0.8583 mL 1.7167 mL 4.2917 mL
10 mM 0.0858 mL 0.4292 mL 0.8583 mL 2.1458 mL
15 mM 0.0572 mL 0.2861 mL 0.5722 mL 1.4306 mL
20 mM 0.0429 mL 0.2146 mL 0.4292 mL 1.0729 mL
25 mM 0.0343 mL 0.1717 mL 0.3433 mL 0.8583 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Fluorescent brightener 220
Cat. No.:
HY-D1171
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