FITC-Dextran (MW 1000) 

FITC-Dextran (MW 1000) is a compound that belongs to the class of fluorescent dyes. It is often used as a tracer molecule in biomedical research to label and track cells or other biological substances. FITC-Dextran consists of fluorescein isothiocyanate (FITC) and dextran, a complex carbohydrate extracted from starch. The combination of the two creates a stable fluorescent tracer that can be observed under a microscope or quantified using specialized detection instruments.

FITC-Dextran (MW 1000) is a fluorescein isothiocyanate (FITC) dextran fluorescent probe (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 1000) can be used as a marker to reveal cell damage caused by heat shock and to study the early and late stages of apoptosis. FITC-Dextran (MW 1000) can also be used to study cell permeability, such as the determination of blood-brain barrier permeability and the degree of blood-brain barrier damage. Storage: Protect from light.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

Intestinal barrier function assay^[5]
1. Starve mice for 4 hours.
2. Orally administer FITC-Dextran MW 20000 to mice (0.6 mg/g).
3. Measure fluorescence intensity (Ex nm/Em 520 nm) within 4 hours.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1000.00 (Average)

Solid

[FITC-Dextran (MW 1000)]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

  [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.  [Content Brief]

  [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.  [Content Brief]

  [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276.  [Content Brief]

  [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282.  [Content Brief]