2. Apoptosis
  3. Ferroptosis
  4. Ferroptosis-IN-22

Ferroptosis-IN-22 is a selective ferroptosis inhibitor by targeting NCOA4 and disrupting its interaction with ferritin with an EC50 of 520 nM and a Kd of 0.78 μM. Ferroptosis-IN-22 has a strong inhibitory activity against ferroptosis induced by multiple ferroptosis inducers (RSL3 (HY-100218A), Erastin (HY-15763), ML210 (HY-100003), FIN56 (HY-103087)), but does not inhibit necrosis induced by H2O2 or apoptosis induced by STS (HY-15141). Ferroptosis-IN-22 effectively ameliorates Concanavalin A (HY-P2149)-induced acute liver injury. Ferroptosis-IN-22 can be used for the study of ferroptosis-related diseases.

For research use only. We do not sell to patients.

Ferroptosis-IN-22

Ferroptosis-IN-22 Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Ferroptosis-IN-22 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ferroptosis-IN-22 is a selective ferroptosis inhibitor by targeting NCOA4 and disrupting its interaction with ferritin with an EC50 of 520 nM and a Kd of 0.78 μM. Ferroptosis-IN-22 has a strong inhibitory activity against ferroptosis induced by multiple ferroptosis inducers (RSL3 (HY-100218A), Erastin (HY-15763), ML210 (HY-100003), FIN56 (HY-103087)), but does not inhibit necrosis induced by H2O2 or apoptosis induced by STS (HY-15141). Ferroptosis-IN-22 effectively ameliorates Concanavalin A (HY-P2149)-induced acute liver injury. Ferroptosis-IN-22 can be used for the study of ferroptosis-related diseases[1].

In Vitro

Ferroptosis-IN-22 (Compound 4k) exhibits anti-ferroptotic activity with EC50 values were 0.515 μM (RSL3) and 2.776 μM (Erastin) in HT-1080 cells, 0.4061 μM (RSL3) and 1.308 μM (Erastin) in 786-O cells, and 0.093 μM (RSL3) and 1.013 μM (Erastin) in MDA-MB-231 cells, and also suppresses ferroptosis induced by ML210 and FIN56[1].
Ferroptosis-IN-22 (4-8 μM) significantly reduced RSL3 or Erastin induced lipid peroxidation in both HT-1080 and 786-O cells and does not have direct antioxidative activity[1].
Ferroptosis-IN-22 (4 μM, 6 h) directly binds to NCOA4, a key receptor protein in ferritin autophagy, disrupting the interaction between NCOA4 and ferritin (FTH1), thereby inhibiting the ferritin autophagy process rather than relying on direct iron chelation in HT-1080 cells[1].
Ferroptosis-IN-22 demonstrates low cardiotoxicity and minimal risk of CYP-mediated drug-drug interactions (DDIs)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ferroptosis-IN-22 Related Antibodies

Western Blot Analysis[1]

Cell Line: HT-1080 cells
Concentration: 4 μM
Incubation Time: 6 h
Result: Significantly reduced the amount of FTH1/FTL co-precipitated with NCOA4.
Parmacokinetics[1]
Species Dose Route Indicator value
Rat 10 mg/kg p.o. T1/2 3.751 h
Rat 5 mg/kg i.v. T1/2 4.009 h
Rat 10 mg/kg p.o. Tmax 2.000 h
Rat 5 mg/kg i.v. Tmax 0.083 h
Rat 10 mg/kg p.o. Cmax 557.082 ng/mL
Rat 5 mg/kg i.v. Cmax 61139.150 ng/mL
Rat 10 mg/kg p.o. AUC0-t 1474.816 ng·h/mL
Rat 5 mg/kg i.v. AUC0-t 30464.355 ng·h/mL
Rat 10 mg/kg p.o. AUC0-∞ 1498.461 ng·h/mL
Rat 5 mg/kg i.v. AUC0-∞ 30509.903 ng·h/mL
Rat 10 mg/kg p.o. MRT0-t 2.961 h
Rat 5 mg/kg i.v. MRT0-t 0.645 h
Rat 10 mg/kg p.o. MRT0-∞ 3.263 h
Rat 5 mg/kg i.v. MRT0-∞ 0.689 h
Rat 10 mg/kg p.o. F 2.421 %
Rat 5 mg/kg i.v. Vss 113.508 mL/kg
Rat 5 mg/kg i.v. Vd 941.525 mL/kg
Rat 5 mg/kg i.v. CL 164.535 mL/h/kg
In Vivo

Ferroptosis-IN-22 (Compound 4k) (5-15 mg/kg, i.p., 2 doses before ConA injection) alleviates Concanavalin A (ConA) (HY-P2149)-Induced acute liver injury by ferroptosis inhibition[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ConA induced acute liver injury model established in C57BL/6J mice[1]
Dosage: 5 and 15 mg/kg
Administration: Intraperitoneal injection (i.p.), 24 hours and 0.5 hours before ConA injection
Result: Decreased serum ALT and AST levels and improved liver tissue injury.
Significantly reduced the mRNA levels of TNF-α and IL-6, two major inflammatory cytokines in the liver.
Effectively reduced the levels of4-HNE and MDA, two lipid peroxidation products, in liver tissues.
Showed no effect on the morphology and the 4-HNE level of other vital organs including kidney, spleen, heart and lung.
Reduced the mRNA expression of ferroptosis marker Ptgs2.
Molecular Weight

393.48

Formula

C27H23NO2
SMILES

OC1=C(C(C)(C)C)C=C(C2=C(C3=CC=CC=C3)C=C(C4=CC=CC=C4)N=C2O5)C5=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ferroptosis-IN-22 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ferroptosis-IN-22FerroptosisFerritinophagyNCOA4 (Nuclear Receptor Coactivator 4)Pseudonatural Product (PNP)Benzofuro[2,3-b]pyridineRhodium-catalyzed C-H activationIron homeostasisLipid peroxidationAcute liver injury (ConA-induced)Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ferroptosis-IN-22
Cat. No.:
HY-175869
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.