FerroOrange 

FerroOrange is a Fe²⁺ selective probe with an excitation wavelength of 543 nm and an emission wavelength of 580 nm. FerroOrange emits fluorescence after binding to intracellular Fe²⁺, thereby achieving specific labeling of Fe²⁺. FerroOrange is used to detect the content and distribution of Fe²⁺ in cells^[1].

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1 µM with HBSS or cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are plated in 96-well microplates.
2.2 Discard the cell culture medium and wash the cells three times with HBSS or serum-free medium.
2.3 Add the 1 µM FerroOrange working solution and incubate for 30 minutes at 37°C
2.4 Using fluorescence microscopy at Ex/Em = 540/585 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

White to off-white

[FerroOrange]

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Cheng Y, et al. Ferristatin II, an Iron Uptake Inhibitor, Exerts Neuroprotection against Traumatic Brain Injury via Suppressing Ferroptosis. ACS Chem Neurosci. 2022 Mar 2;13(5):664-675.  [Content Brief]