FDGlcU 

FDGlcU can be used as a fluorescent probe for non-invasively image with a high level of fluorescent activity. FDGlcU is non-fluorescent when the fluorescein is conjugated with two mono-glucuronides (Ex/Em=480/514 nm)^[1].

FDGlcU (0.5 μg/mL; 12 h) shows enzymatic activity and inhibits βG activity in live bacteria^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FDGlcU (7.3 μM/kg) can be used for in vivo time-lapse imaging of bacterial βG activity^[1].
FDGlcU can be used for real-time imaging in vivo^[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Whole-body imaging for intestinal βG activity^[1]:
1. On the day of imaging, gavage mice with 50 μL of DDW, FDGlcU (7.3 μM/kg), and fluorescein (7.3 μM/kg).
2. Anesthetize mice with isoflurane and whole-body optical images using an IVIS spectrum optical imaging system with a GFP filter set.
3. In biodistribution study, mice are sacrificed 3 hour after gavaged with FDGlcU or fluorescein (λex =480 nm, λem =514 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

893.63

C₃₉H₂₈F₅NO₁₈

209540-67-0

Solid

Light yellow to yellow

O=C1OC2(C3=CC=C(O[C@@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)C(O)=O)C=C3OC5=CC(O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O)O)O)C(O)=O)=CC=C52)C7=CC=C(NC(C8=C(C(F)=C(C(F)=C8F)F)F)=O)C=C71

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Chen M, et al. Real-time imaging of intestinal bacterial β-glucuronidase activity by hydrolysis of a fluorescent probe. Sci Rep. 2017 Jun 9;7(1):3142.  [Content Brief]