FAM amine, 6-isomer

Cat. No.: HY-D1336 Purity: 98.12%: Data Sheet
COA

SDS
Handling Instructions
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FAM amine, 6-isomer is a fluorescein derivative with an amine group and contains an isomer of the fluorophore. Can be used to modify biomolecules through enzymatic transamination. Its fatty amine groups can also react with electrophiles such as activated esters. The amine can also be conjugated to carbonyl compounds (aldehydes and ketones) by reductive amination.

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FAM amine, 6-isomer Chemical Structure

CAS No. : 2183440-42-6

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Molecular Weight

510.97

Formula

C₂₇H₂₇ClN₂O₆

CAS No.

2183440-42-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1OC2(C3=CC(C(NCCCCCCN)=O)=CC=C13)C4=CC=C(C=C4OC5=CC(O)=CC=C52)O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (97.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9571 mL	9.7853 mL	19.5706 mL
5 mM	0.3914 mL	1.9571 mL	3.9141 mL
10 mM	0.1957 mL	0.9785 mL	1.9571 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.12%

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Data Sheet (270 KB) SDS (252 KB)

COA (250 KB) HNMR (316 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)

References

[1]. Hung S C, Ju J, Mathies R A, et al. Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers1[J]. Analytical biochemistry, 1996, 243(1): 15-27.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9571 mL	9.7853 mL	19.5706 mL	48.9266 mL
	5 mM	0.3914 mL	1.9571 mL	3.9141 mL	9.7853 mL
	10 mM	0.1957 mL	0.9785 mL	1.9571 mL	4.8927 mL
	15 mM	0.1305 mL	0.6524 mL	1.3047 mL	3.2618 mL
	20 mM	0.0979 mL	0.4893 mL	0.9785 mL	2.4463 mL
	25 mM	0.0783 mL	0.3914 mL	0.7828 mL	1.9571 mL
	30 mM	0.0652 mL	0.3262 mL	0.6524 mL	1.6309 mL
	40 mM	0.0489 mL	0.2446 mL	0.4893 mL	1.2232 mL
	50 mM	0.0391 mL	0.1957 mL	0.3914 mL	0.9785 mL
	60 mM	0.0326 mL	0.1631 mL	0.3262 mL	0.8154 mL
	80 mM	0.0245 mL	0.1223 mL	0.2446 mL	0.6116 mL