Ethylenethiourea

Cat. No.: HY-W010593 Purity: ≥98.0%: Data Sheet
SDS

COA
Handling Instructions
FAQs
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Ethylenethiourea is a degradation product of the ethylenebisthiocarbamate group of fungicides. Ethylenethiourea is tumorigenic and teratogenic. Ethylenethiourea is orally active.

For research use only. We do not sell to patients.

Ethylenethiourea Chemical Structure

CAS No. : 96-45-7

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 g	In-stock	Estimated Time of Arrival: December 31
250 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Other Forms of Ethylenethiourea:

Ethylenethiourea-d₄ Get quote
Ethylenethiourea (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Ethylenethiourea is a degradation product of the ethylenebisthiocarbamate group of fungicides. Ethylenethiourea is tumorigenic and teratogenic. Ethylenethiourea is orally active^[1]^[2].

In Vitro

Ethylenethiourea is stable at elevated temperature over a wide pH range^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ethylenethiourea (10-80 mg/kg; p.o.) is teratogenic in rats^[1].
Ethylenethiourea (5-500 ppm; in the diet; 12 month) induces thyroid carcinomas in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Virgin Wistar female rats^[1]
Dosage:	0, 5, 10, 20, 40 or 80 mg/kg
Administration:	Oral administration, from 21-42 days before conception to pregnancy day 15, and on days 6-15 or 7-20 of pregnancy
Result:	Induced meningoencephalocele, meningorrhagia, meningorrhea, hydrocephalus, obliterated neural canal, abnormal pelvic limb posture with equinovarus, and short or kinky tail after 10 mg/kg or more.

Molecular Weight

102.16

Formula

C₃H₆N₂S

CAS No.

96-45-7

Appearance

Solid

Color

White to off-white

SMILES

S=C1NCCN1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (244.71 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	9.7886 mL	48.9428 mL	97.8857 mL
5 mM	1.9577 mL	9.7886 mL	19.5771 mL
10 mM	0.9789 mL	4.8943 mL	9.7886 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (48.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (48.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (48.94 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (557 KB)

COA (249 KB) HNMR (113 KB)

Handling Instructions (2659 KB)

References

[1]. Khera KS. Ethylenethiourea: teratogenicity study in rats and rabbits. Teratology. 1973 Jun;7(3):243-52. [Content Brief]

[2]. Graham SL, et al. Effects of one-year administration of ethylenethiourea upon the thyroid of the rat. J Agric Food Chem. 1973 May-Jun;21(3):324-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	9.7886 mL	48.9428 mL	97.8857 mL	244.7142 mL
	5 mM	1.9577 mL	9.7886 mL	19.5771 mL	48.9428 mL
	10 mM	0.9789 mL	4.8943 mL	9.7886 mL	24.4714 mL
	15 mM	0.6526 mL	3.2629 mL	6.5257 mL	16.3143 mL
	20 mM	0.4894 mL	2.4471 mL	4.8943 mL	12.2357 mL
	25 mM	0.3915 mL	1.9577 mL	3.9154 mL	9.7886 mL
	30 mM	0.3263 mL	1.6314 mL	3.2629 mL	8.1571 mL
	40 mM	0.2447 mL	1.2236 mL	2.4471 mL	6.1179 mL
	50 mM	0.1958 mL	0.9789 mL	1.9577 mL	4.8943 mL
	60 mM	0.1631 mL	0.8157 mL	1.6314 mL	4.0786 mL
	80 mM	0.1224 mL	0.6118 mL	1.2236 mL	3.0589 mL
	100 mM	0.0979 mL	0.4894 mL	0.9789 mL	2.4471 mL