Ethyl phenylglyoxylate (Synonyms: Ethyl benzoylformate; Phenylglyoxylic acid ethyl ester)

Cat. No.: HY-W016618 Purity: 98.31%: Data Sheet
COA

SDS
Handling Instructions
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Ethyl phenylglyoxylate (Ethyl benzoylformate), the ethyl ester of phenylglyoxylic acid, is used as a synthetic reagent. Ethyl phenylglyoxylate is also a poor substrate but a potent inhibitor of chicken liver carboxylesterase. Additionally, Ethyl phenylglyoxylate exhibits photoreactivity, where its excited triplet carbonyl can initiate intermolecular hydrogen abstraction, radical coupling and cross-linking reactions.

For research use only. We do not sell to patients.

Ethyl phenylglyoxylate Chemical Structure

CAS No. : 1603-79-8

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Other Forms of Ethyl phenylglyoxylate:

Ethyl phenylglyoxylate (Standard) Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Ethyl phenylglyoxylate undergoes photoreduction to primarily yield Diethyl 2,3-dihydroxy-2,3-diphenylsuccinate in the presence of hydrogen donors (e.g., isopropyl alcohol), while additional adducts are formed in the presence of electron donors (e.g., thioethers, pyridine)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

178.19

Synonyms

Ethyl benzoylformate; Phenylglyoxylic acid ethyl ester

Formula

C₁₀H₁₀O₃

CAS No.

1603-79-8

Appearance

Liquid (Density: 1.122 g/cm³)

Color

Colorless to light yellow

SMILES

CCOC(C(C1=CC=CC=C1)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (561.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6120 mL	28.0599 mL	56.1199 mL
5 mM	1.1224 mL	5.6120 mL	11.2240 mL
10 mM	0.5612 mL	2.8060 mL	5.6120 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.31%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (241 KB) RP-HPLC (225 KB) MS (176 KB)

Handling Instructions (2659 KB)

References

[1]. Shengkui Hu, et al. Photochemically Active Polymers Containing Pendant Ethyl Phenylglyoxylate. Macromolecules 1998, 31, 2, 322–327.

[2]. Hu S, et al. Photoreduction of ethyl phenylglyoxylate. Journal of Photochemistry and Photobiology A: Chemistry, 1998, 114(2): 103-108.

[3]. Berndt M C, et al. Ethyl phenylglyoxylate, a simultaneous inhibitor and substrate of chicken liver carboxylesterase (EC 3.1. 1.1). Enzyme-catalyzed fragmentation of (E)-benzil monoxime O-2, 4-dinitrophenyl ether. Journal of the American Chemical Society, 1977, 99(25): 8334-8335.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6120 mL	28.0599 mL	56.1199 mL	140.2997 mL
	5 mM	1.1224 mL	5.6120 mL	11.2240 mL	28.0599 mL
	10 mM	0.5612 mL	2.8060 mL	5.6120 mL	14.0300 mL
	15 mM	0.3741 mL	1.8707 mL	3.7413 mL	9.3533 mL
	20 mM	0.2806 mL	1.4030 mL	2.8060 mL	7.0150 mL
	25 mM	0.2245 mL	1.1224 mL	2.2448 mL	5.6120 mL
	30 mM	0.1871 mL	0.9353 mL	1.8707 mL	4.6767 mL
	40 mM	0.1403 mL	0.7015 mL	1.4030 mL	3.5075 mL
	50 mM	0.1122 mL	0.5612 mL	1.1224 mL	2.8060 mL
	60 mM	0.0935 mL	0.4677 mL	0.9353 mL	2.3383 mL
	80 mM	0.0701 mL	0.3507 mL	0.7015 mL	1.7537 mL
	100 mM	0.0561 mL	0.2806 mL	0.5612 mL	1.4030 mL

Ethyl phenylglyoxylate Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethyl phenylglyoxylate1603-79-8Ethyl benzoylformate Phenylglyoxylic acid ethyl esterDrug IntermediateBiochemical Assay ReagentsCarboxylesterase (CES)Drug IintermediateCarboxylesteraseCarboxylic-ester Hydrolasesynthetic reagentchicken liver carboxylesteraseInhibitorinhibitorinhibit

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