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  4. EGTA tetrasodium

EGTA tetrasodium is a specific calcium ion chelator. EGTA tetrasodium has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA tetrasodium significantly inhibits the substrate adherence capacity of inflammatory macrophages.

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EGTA tetrasodium

EGTA tetrasodium Chemical Structure

CAS No. : 13368-13-3

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Based on 18 publication(s) in Google Scholar

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EGTA tetrasodium Related Antibodies

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Description

EGTA tetrasodium is a specific calcium ion chelator. EGTA tetrasodium has an apparent calcium dissociation constant (Kd) of 60.5 nM at physiological pH (7.4) and has very high specificity for Ca2+ over Mg2+ (Mg2+ Kd 1-10 mM). EGTA tetrasodium significantly inhibits the substrate adherence capacity of inflammatory macrophages[1][2].

In Vitro

EGTA tetrasodium, proposed as endodontic irrigant, decreases substrate adherence capacity of inflammatory macrophages in a time- and dose-dependent manner. The EGTA tetrasodium concentration that causes an IC50 is 202 mM. Chelators react with calcium ions in the hydroxyapatite crystals to produce a metallic chelate. Removal of calcium ions from the dentine makes the dentinal tissue softer, especially the hydroxyapatite-rich peritubular dentin and increases the diameter of exposed dentinal tubules[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EGTA tetrasodium Related Antibodies
Molecular Weight

468.28

Formula

C14H20N2Na4O10
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O[Na])CN(CC(O[Na])=O)CCOCCOCCN(CC(O[Na])=O)CC(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (213.55 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1355 mL 10.6774 mL 21.3547 mL
5 mM 0.4271 mL 2.1355 mL 4.2709 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.1355 mL 10.6774 mL 21.3547 mL 53.3869 mL
5 mM 0.4271 mL 2.1355 mL 4.2709 mL 10.6774 mL
10 mM 0.2135 mL 1.0677 mL 2.1355 mL 5.3387 mL
15 mM 0.1424 mL 0.7118 mL 1.4236 mL 3.5591 mL
20 mM 0.1068 mL 0.5339 mL 1.0677 mL 2.6693 mL
25 mM 0.0854 mL 0.4271 mL 0.8542 mL 2.1355 mL
30 mM 0.0712 mL 0.3559 mL 0.7118 mL 1.7796 mL
40 mM 0.0534 mL 0.2669 mL 0.5339 mL 1.3347 mL
50 mM 0.0427 mL 0.2135 mL 0.4271 mL 1.0677 mL
60 mM 0.0356 mL 0.1780 mL 0.3559 mL 0.8898 mL
80 mM 0.0267 mL 0.1335 mL 0.2669 mL 0.6673 mL
100 mM 0.0214 mL 0.1068 mL 0.2135 mL 0.5339 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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EGTA tetrasodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EGTA tetrasodium13368-13-3Biochemical Assay ReagentscalciumionchelatorendodonticirrigantinflammatorymacrophagesInhibitorinhibitorinhibit

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