2. PROTAC Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease NF-κB
  3. PROTACs Pyroptosis Reactive Oxygen Species (ROS) Caspase
  4. DeFer-2

DeFer-2 is a ferritin PROTAC degrader (Kd = 17.1 μM). DeFer-2 induces ferritin degradation, triggering caspase 3-GSDME-mediated pyroptosis in cancer cells through free iron accumulation and elevated ROS. DeFer-2 significantly inhibits tumor growth and prolongs survival in mice bearing B16F10 subcutaneous tumors. DeFer-2 can be used to study melanoma. (Pink: Oleic acid: HY-N1446, Blue: (S,R,S)-AHPC: HY-125845, Black: γ-Aminobutyric acid: HY-N0067, Blue + Black: (S,R,S)-AHPC-C3-NH2: HY-130711).

For research use only. We do not sell to patients.

DeFer-2

DeFer-2 Chemical Structure

CAS No. : 3058911-16-0

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

DeFer-2 Related Antibodies

Top Publications Citing Use of Products

View All PROTACs Isoform Specific Products:

View All Isoforms
von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DeFer-2 is a ferritin PROTAC degrader (Kd = 17.1 μM). DeFer-2 induces ferritin degradation, triggering caspase 3-GSDME-mediated pyroptosis in cancer cells through free iron accumulation and elevated ROS. DeFer-2 significantly inhibits tumor growth and prolongs survival in mice bearing B16F10 subcutaneous tumors. DeFer-2 can be used to study melanoma. (Pink: Oleic acid: HY-N1446, Blue: (S,R,S)-AHPC: HY-125845, Black: γ-Aminobutyric acid: HY-N0067, Blue + Black: (S,R,S)-AHPC-C3-NH2: HY-130711)[1].

IC50 & Target[1]

VHL

 

Caspase 3

 

In Vitro

DeFer-2 (0-5 μM, 0-24 h) reduces the ferritin expression by forming a ternary complex with ferritin and VHL, induces disruption of redox and mitochondrial homeostasis in B16F10 cells[1].
DeFer-2 (5 μM, 8-24 h) initiates caspase 3-GSDME-mediated pyroptosis through free iron accumulation and ROS elevation in B16F10 cells[1].
DeFer-2 (24-48 h) causes severe cytotoxicity to B16F10 cells with IC50s of 3.1 and 1.5 μM for 24 and 48 h, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DeFer-2 Related Antibodies

Western Blot Analysis[1]

Cell Line: B16F10 cells
Concentration: 0, 0.008, 0.04, 0.2, 0.5, 1, 5 μM
Incubation Time: 0 h, 4 h , 8 h, 12 h, 16 h, 24 h
Result: Reduced the ferritin expression.
Reduced the ferritin expression when as a nanoparticle albumin-DeFer-2.

Immunofluorescence[1]

Cell Line: B16F10 cells
Concentration: 5 μM
Incubation Time: 16 h
Result: Increased ROS levels.
In Vivo

DeFer-2 (nanoparticle Albumin-DeFer-2, 10 mg/kg, i.v., every other day, 8 days) inhibits tumor growth and prolongs survival time in B16F10 tumor mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B16F10 tumor (1 × 106) mice (8-week-old, C57BL/6, male) model[1]
Dosage: 10 mg/kg; in the form of nanoparticle albumin-DeFer-2
Administration: i.v., every other day, 8 days
Result: Inhibited tumor growth and prolonged survival time.
Did not cause systemic toxicity.
Molecular Weight

780.11

Formula

C44H69N5O5S
CAS No.
SMILES

CC1=C(SC=N1)C2=CC=C(C=C2)CNC([C@H](C[C@@H](O)C3)N3C([C@H](C(C)(C)C)NC(CCCNC(CCCCCCC/C=C\CCCCCCCC)=O)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

DeFer-2 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DeFer-23058911-16-0DeFer2DeFer 2PROTACsPyroptosisReactive Oxygen Species (ROS)CaspaseDegradermelanomaB16F10 cellspyroptosisB16F10 tumor miceInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
DeFer-2
Cat. No.:
HY-175381
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.