2. Apoptosis
  3. Apoptosis
  4. DC-5163

DC-5163 is a potent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhibitor with an IC50 of 176.3 nM and a Kd of 3.192 μM. DC-5163 can inhibit glycolysis pathway partially. DC-5163 also selectively inhibits cancer cell proliferation and induces apoptosis.

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DC-5163 Chemical Structure

DC-5163 Chemical Structure

CAS No. : 897771-47-0

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Based on 1 publication(s) in Google Scholar

DC-5163 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE DC-5163

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  • Purity & Documentation

  • References

  • Customer Review

Description

DC-5163 is a potent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inhibitor with an IC50 of 176.3 nM and a Kd of 3.192 μM. DC-5163 can inhibit glycolysis pathway partially. DC-5163 also selectively inhibits cancer cell proliferation and induces apoptosis[1].

IC50 & Target

IC50: 176.3 nM (Glyceraldehyde-3-phosphate dehydrogenase (GAPDH))[1]
Kd: 3.192 μM (GAPDH)[1]
In Vitro

DC-5163 (100 μM; 48 hours; MDA-MB-231 cells) treatment clearly induces the apoptosis of MDA-MB-231 cells. DC-5163 also significantly reduces glucose uptake, lactate production, and 18F-FDG uptake rate compared to controls in MDA-MB-231cells[1].
DC-5163 treatment at 25 μM inhibits GAPDH activity in BT-549 cells, MCF7 cells, HCT116 cells, MDA-MB-231 cells and A549 cells after 48 hours[1].
DC-5163 inhibits MDA-MB-231 cell proliferation with an IC50 of 99.22 μM at 48 hours and 52.09 μM at 72 hours[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DC-5163 Related Antibodies

Apoptosis Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 100 μM
Incubation Time: 48 hours
Result: Clearly induced the apoptosis of MDA-MB-231 cells.
Molecular Weight

361.89

Formula

C18H20ClN3OS
CAS No.
Appearance

Solid

SMILES

S=C(N1CCN(CC2=CC=CC=C2)C1)NC3=CC=C(OC)C(Cl)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 300 mg/mL (828.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7633 mL 13.8164 mL 27.6327 mL
5 mM 0.5527 mL 2.7633 mL 5.5265 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 7.5 mg/mL (20.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7633 mL 13.8164 mL 27.6327 mL 69.0818 mL
5 mM 0.5527 mL 2.7633 mL 5.5265 mL 13.8164 mL
10 mM 0.2763 mL 1.3816 mL 2.7633 mL 6.9082 mL
15 mM 0.1842 mL 0.9211 mL 1.8422 mL 4.6055 mL
20 mM 0.1382 mL 0.6908 mL 1.3816 mL 3.4541 mL
25 mM 0.1105 mL 0.5527 mL 1.1053 mL 2.7633 mL
30 mM 0.0921 mL 0.4605 mL 0.9211 mL 2.3027 mL
40 mM 0.0691 mL 0.3454 mL 0.6908 mL 1.7270 mL
50 mM 0.0553 mL 0.2763 mL 0.5527 mL 1.3816 mL
60 mM 0.0461 mL 0.2303 mL 0.4605 mL 1.1514 mL
80 mM 0.0345 mL 0.1727 mL 0.3454 mL 0.8635 mL
100 mM 0.0276 mL 0.1382 mL 0.2763 mL 0.6908 mL
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DC-5163 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DC-5163897771-47-0DC5163DC 5163ApoptosisGlycolysisanticancerlacticacidanti-proliferationglucoseapoptosisGAPDHInhibitorinhibitorinhibit

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