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  2. Fluorescent Dye
  3. Cyanine5.5 NHS ester tetrafluoroborate

Cyanine5.5 NHS ester tetrafluoroborate belongs to the cyanine dye series and is a common fluorescent marker for biomolecules that can interact with biomolecules. Cyanine dyes may also bind to double-helical DNA through intercalation and exhibit enhanced fluorescence upon binding.

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Cyanine5.5 NHS ester tetrafluoroborate

Cyanine5.5 NHS ester tetrafluoroborate Chemical Structure

CAS No. : 2375105-86-3

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Based on 1 publication(s) in Google Scholar

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Description

Cyanine5.5 NHS ester tetrafluoroborate belongs to the cyanine dye series and is a common fluorescent marker for biomolecules that can interact with biomolecules. Cyanine dyes may also bind to double-helical DNA through intercalation and exhibit enhanced fluorescence upon binding.

Molecular Weight

767.66

Formula

C44H46BF4N3O4

CAS No.
Appearance

Solid

SMILES

O=C(CCCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C3=[N+](C)C4=C(C3(C)C)C5=C(C=CC=C5)C=C4)C6=C(C=CC=C6)C=C2)ON7C(CCC7=O)=O.[F-][B+3]([F-])([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (65.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3027 mL 6.5133 mL 13.0266 mL
5 mM 0.2605 mL 1.3027 mL 2.6053 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3027 mL 6.5133 mL 13.0266 mL 32.5665 mL
5 mM 0.2605 mL 1.3027 mL 2.6053 mL 6.5133 mL
10 mM 0.1303 mL 0.6513 mL 1.3027 mL 3.2567 mL
15 mM 0.0868 mL 0.4342 mL 0.8684 mL 2.1711 mL
20 mM 0.0651 mL 0.3257 mL 0.6513 mL 1.6283 mL
25 mM 0.0521 mL 0.2605 mL 0.5211 mL 1.3027 mL
30 mM 0.0434 mL 0.2171 mL 0.4342 mL 1.0856 mL
40 mM 0.0326 mL 0.1628 mL 0.3257 mL 0.8142 mL
50 mM 0.0261 mL 0.1303 mL 0.2605 mL 0.6513 mL
60 mM 0.0217 mL 0.1086 mL 0.2171 mL 0.5428 mL
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Cyanine5.5 NHS ester tetrafluoroborate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cyanine5.5 NHS ester tetrafluoroborate
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HY-D1322
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