Crystal violet, IND (0.0-2.0)

Name: Crystal violet, IND (0.0-2.0)
Brand: MedChemExpress
SKU: HY-W110794
Rating: 4.5 (1 reviews)

Cat. No.: HY-W110794: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Crystal violet, IND (0.0-2.0) is a specialized indicator suitable for strongly acidic systems, which reflects changes in pH within the range of 0.0-2.0 through color changes. Crystal violet, IND (0.0-2.0) is widely used in acid-base titration, chemical analysis, and industrial testing fields.

For research use only. We do not sell to patients.

Crystal violet, IND (0.0-2.0) Chemical Structure

CAS No. : 548-62-9

Size	Stock	Quantity
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
H9c2	CC₅₀	1.5 μM Compound: Gentian violet	Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay	[PMID: 29031078]
J774	CC₅₀	0.45 μM Compound: Gentian violet	Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay	[PMID: 29031078]
J774	CC₅₀	0.45 μM Compound: GV	Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay	[PMID: 26549870]
J774	CC₅₀	0.8 μM Compound: Gentian violet	Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by Alamar Blue assay Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by Alamar Blue assay	[PMID: 32364737]
J774	CC₅₀	4.1 μM Compound: GV	Cytotoxicity against BALB/c mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamarBlue assay Cytotoxicity against BALB/c mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamarBlue assay	[PMID: 29523468]
RAW264.7	IC₅₀	1.1 μM Compound: Gentian violet	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in IFN-gamma and LPS-induced nitric oxide production after 24 hrs by ELISA Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in IFN-gamma and LPS-induced nitric oxide production after 24 hrs by ELISA	[PMID: 35998343]

In Vitro

Reference procedures for the determination of cationic surfactants by photometric titration^[1]:
1. Take an appropriate amount of cationic surfactant solution into a beaker, add deionized water, 0.5 mL of 1 mM Crystal violet, IND, and 4 mL of pH 2.2 buffer solution, then dilute to 40 mL.
2. Use a 0.1 mM potassium poly(vinyl sulfate) (PVSK) solution as the titrant and add it to the sample solution in successive portions.
3. After each addition, measure the absorbance of the solution at 590 nm using a spectrophotometer.
4. Before titration, the Crystal violet, IND does not react with the cationic surfactant, and the absorbance corresponds to the indicator itself. Before the equivalence point, the absorbance decreases slightly due to dilution by the titrant and ion association.
5. At the end point, the association of the Crystal violet, IND with the titrant causes a sharp decrease in its concentration, leading to a sudden drop in absorbance. The break point of the titration curve is taken as the end point.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

407.98

Formula

C₂₅H₃₀ClN₃

CAS No.

548-62-9

Appearance

Solid

Color

Green to dark green

SMILES

C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=C(N(C)C)C=C3.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (122.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4511 mL	12.2555 mL	24.5110 mL
5 mM	0.4902 mL	2.4511 mL	4.9022 mL
10 mM	0.2451 mL	1.2256 mL	2.4511 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Select Batch:

Data Sheet (273 KB) SDS (922 KB)

COA (315 KB) HNMR (119 KB) MS (77 KB)

Handling Instructions (2659 KB)

References

[1]. Masadome T. Determination of cationic surfactants by a photometric titration method with crystal violet as a color indicator. Analytical letters, 2004, 37(3): 499-506.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4511 mL	12.2555 mL	24.5110 mL	61.2775 mL
	5 mM	0.4902 mL	2.4511 mL	4.9022 mL	12.2555 mL
	10 mM	0.2451 mL	1.2256 mL	2.4511 mL	6.1278 mL
	15 mM	0.1634 mL	0.8170 mL	1.6341 mL	4.0852 mL
	20 mM	0.1226 mL	0.6128 mL	1.2256 mL	3.0639 mL
	25 mM	0.0980 mL	0.4902 mL	0.9804 mL	2.4511 mL
	30 mM	0.0817 mL	0.4085 mL	0.8170 mL	2.0426 mL
	40 mM	0.0613 mL	0.3064 mL	0.6128 mL	1.5319 mL
	50 mM	0.0490 mL	0.2451 mL	0.4902 mL	1.2256 mL
	60 mM	0.0409 mL	0.2043 mL	0.4085 mL	1.0213 mL
	80 mM	0.0306 mL	0.1532 mL	0.3064 mL	0.7660 mL
	100 mM	0.0245 mL	0.1226 mL	0.2451 mL	0.6128 mL