Copper sulfate pentahydrate, 99.9%

Cat. No.: HY-Y1881A: Data Sheet
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Copper sulfate pentahydrate, 99% is a biochemical reagent. Copper sulfate pentahydrate, 99% reduces the production of ROS and the expression levels of MyD88 as well as c-Rel genes. Copper sulfate pentahydrate, 99% decreases the activities of T-SOD, CAT, and GSH, increases the activities of caspase-3, caspase-8, and caspase-9. Copper sulfate pentahydrate, 99% is cytotoxic to various cells. Copper sulfate pentahydrate, 99% has antioxidant activity. Copper sulfate pentahydrate, 99% can be used in the research of diabetes, Parkinson's disease and DMBA (HY-W011845)-induced tumors.

For research use only. We do not sell to patients.

Copper sulfate pentahydrate, 99.9% Chemical Structure

CAS No. : 7758-99-8

Size	Stock	Quantity
Free Sample (0.1 - 0.5 mg)	Apply Now
250 mg	In-stock	Estimated Time of Arrival: December 31
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Other Forms of Copper sulfate pentahydrate, 99.9%:

Copper sulfate pentahydrate, for cell culture, 98% In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]^[3]^[4]

Caspase-3

Caspase-9

Caspase-8

In Vitro

Copper sulfate pentahydrate (6.25-200 mg/mL; 24 h), 99.9% significantly decreases the cell viability of three human intestinal epithelial cell lines (HCT116, HT-29 and SW480)^[1].
Copper sulfate (2.4 mg Cu/L; 24 h) pentahydrate, 99.9% significantly decreases the viability of primary hepatocytes of Epinephelus coioides^[2].
Copper sulfate (5-100 µM) pentahydrate, 99.9% has no effect on CHO cell growth or antibody production, with a slight dose-dependent decrease in culture viability^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:	primary hepatocytes of Epinephelus coioides
Concentration:	2.4 mg Cu/L
Incubation Time:	24 h
Result:	Increased the percentages of apoptosis and necrosis. Increased the activities of caspase-3, caspase-8, and caspase-9.

In Vivo

Copper sulfate (20-25 μg/0.2 mL; i.e.; every other day for 30 days) pentahydrate, 99% prevents diabetes and preserves pancreatic β-cell function in STZ (HY-13753)-induced diabetic C57BL6 female mice^[5].
Copper sulfate (2.5 mg/kg; i.p.; 16/24 h before MPP⁺) pentahydrate, 99% blocks MPP⁺-induced striatal lipid peroxidation and dopamine depletion in mice^[6].
Copper sulfate (approximately 50 mg Cu²⁺/liter in drinking water) pentahydrate, 99% delays DMBA (HY-W011845)-induced ovarian granulosa cell tumor development in C57BL/6J female mice^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

249.69

Formula

CuH₁₀O₉S

CAS No.

7758-99-8

Appearance

Solid

Color

Light blue to blue

SMILES

O=S([O-])([O-])=O.[H]O[H].[H]O[H].[H]O[H].[H]O[H].[H]O[H].[Cu+2]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature, keep dry and cool

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (400.50 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0050 mL	20.0248 mL	40.0497 mL
5 mM	0.8010 mL	4.0050 mL	8.0099 mL
10 mM	0.4005 mL	2.0025 mL	4.0050 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (274 KB) SDS (758 KB)

COA (232 KB)

Handling Instructions (2659 KB)

References

[1]. Feyzi A, et al. Copper sulfate pentahydrate reduced epithelial cytotoxicity induced by lipopolysaccharide from enterogenic bacteria. Biomed Pharmacother. 2017 May;89:454-461. [Content Brief]

[2]. Wang T, et al. Copper Nanoparticles and Copper Sulphate Induced Cytotoxicity in Hepatocyte Primary Cultures of Epinephelus coioides. PLoS One. 2016 Feb 18;11(2):e0149484. [Content Brief]

[3]. Chaderjian WB, et al. Effect of copper sulfate on performance of a serum-free CHO cell culture process and the level of free thiol in the recombinant antibody expressed. Biotechnol Prog. 2005 Mar-Apr;21(2):550-3. [Content Brief]

[4]. NAKASHIMA, K. et al.Membrane protein cross-linking is an early step in the pathogenesis of copper-induced hemolytic </b><b>anemia </b> Biomedical Research, 1(6), 548–551.

[5]. Sitasawad S, et al. Beneficial effect of supplementation with copper sulfate on STZ-diabetic mice (IDDM). Diabetes Res Clin Pract. 2001 May;52(2):77-84. [Content Brief]

[6]. Alcaraz-Zubeldia M, et al. Neuroprotective effect of acute and chronic administration of copper (II) sulfate against MPP+ neurotoxicity in mice. Neurochem Res. 2001 Jan;26(1):59-64. [Content Brief]

[7]. Burki HR, et al. Effect of oral copper sulfate on 7,12-dimethylbenz(alpha)anthracene carcinogenesis in mice. Br J Cancer. 1969 Sep;23(3):591-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.0050 mL	20.0248 mL	40.0497 mL	100.1242 mL
	5 mM	0.8010 mL	4.0050 mL	8.0099 mL	20.0248 mL
	10 mM	0.4005 mL	2.0025 mL	4.0050 mL	10.0124 mL
	15 mM	0.2670 mL	1.3350 mL	2.6700 mL	6.6749 mL
	20 mM	0.2002 mL	1.0012 mL	2.0025 mL	5.0062 mL
	25 mM	0.1602 mL	0.8010 mL	1.6020 mL	4.0050 mL
	30 mM	0.1335 mL	0.6675 mL	1.3350 mL	3.3375 mL
	40 mM	0.1001 mL	0.5006 mL	1.0012 mL	2.5031 mL
	50 mM	0.0801 mL	0.4005 mL	0.8010 mL	2.0025 mL
	60 mM	0.0667 mL	0.3337 mL	0.6675 mL	1.6687 mL
	80 mM	0.0501 mL	0.2503 mL	0.5006 mL	1.2516 mL
	100 mM	0.0400 mL	0.2002 mL	0.4005 mL	1.0012 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.