2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. Coelenterazine h hydrochloride

Coelenterazine h hydrochloride  (Synonyms: 2-Deoxycoelenterazine hydrochloride; CLZN-h hydrochloride)

Cat. No.: HY-D1024A Purity: 98.02%
SDS
COA
Handling Instructions Technical Support

Coelenterazine h (2-Deoxycoelenterazine) hydrochloride, a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h hydrochloride is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations (Ex/Em = 437/466 nm).

For research use only. We do not sell to patients.

Coelenterazine h hydrochloride Chemical Structure

Coelenterazine h hydrochloride Chemical Structure

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Coelenterazine h hydrochloride:

Coelenterazine h hydrochloride Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Coelenterazine h hydrochloride

View All Calcium Channel Isoform Specific Products:

View All Isoforms
N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Coelenterazine h (2-Deoxycoelenterazine) hydrochloride, a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h hydrochloride is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations (Ex/Em = 437/466 nm)[1][2][3][4].

In Vitro

1. Solution preparation[4]
1.1 Preparation of stock solution
Solvent: methanol or ethanol
Concentration: 10 mM (optimized according to the experiment).
1.2 Preparation of working solution
Dilute to 10 µM with PBS or cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Hela cells are transfected with BRAC or G5A.
2.2 Add 10 µM Coelenterazine h hydrochloride into the cell plate.
2.3 Incubate the dye-loading plate in a cell incubator for 1-4 h (optimized according to the experiment).
2.4 Fluorescence microscopy detection (Ex/Em = 437/466 nm).
Coelenterazine h (1-10 μM) hydrochloride can be used as the luminescent substrate for RLuc8[4].
In the measurements of Ca2+ binding kinetics of BRAC, emission intensity of Venus (530 nm) from BRAC are monitored at 1 kHz just after rapid mixing of 5 nM BRAC protein with 20 μM coelenterazine-h hydrochloride in various concentration of Ca2+ buffer[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coelenterazine h hydrochloride Related Antibodies
Molecular Weight

443.92

Formula

C26H22ClN3O2
Appearance

Solid

Color

Light yellow to orange

SMILES

OC(C=C1)=CC=C1C2=CN(C(C(CC3=CC=CC=C3)=N4)=O)C4=C(N2)CC5=CC=CC=C5.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

Ethanol : ≥ 50 mg/mL (112.63 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2527 mL 11.2633 mL 22.5266 mL
5 mM 0.4505 mL 2.2527 mL 4.5053 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

    Solubility: 1.25 mg/mL (2.82 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 2.2527 mL 11.2633 mL 22.5266 mL 56.3165 mL
5 mM 0.4505 mL 2.2527 mL 4.5053 mL 11.2633 mL
10 mM 0.2253 mL 1.1263 mL 2.2527 mL 5.6316 mL
15 mM 0.1502 mL 0.7509 mL 1.5018 mL 3.7544 mL
20 mM 0.1126 mL 0.5632 mL 1.1263 mL 2.8158 mL
25 mM 0.0901 mL 0.4505 mL 0.9011 mL 2.2527 mL
30 mM 0.0751 mL 0.3754 mL 0.7509 mL 1.8772 mL
40 mM 0.0563 mL 0.2816 mL 0.5632 mL 1.4079 mL
50 mM 0.0451 mL 0.2253 mL 0.4505 mL 1.1263 mL
60 mM 0.0375 mL 0.1877 mL 0.3754 mL 0.9386 mL
80 mM 0.0282 mL 0.1408 mL 0.2816 mL 0.7040 mL
100 mM 0.0225 mL 0.1126 mL 0.2253 mL 0.5632 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Coelenterazine h hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coelenterazine h2-DeoxycoelenterazineCLZN-hCalcium ChannelCa2+ channelsCa channelsLuminescent SubstratecalciumInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Coelenterazine h hydrochloride
Cat. No.:
HY-D1024A
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.