2. Apoptosis Metabolic Enzyme/Protease Anti-infection
  3. Apoptosis Endogenous Metabolite Bacterial
  4. Citric acid tripotassium hydrate

Citric acid tripotassium hydrate  (Synonyms: Potassium citrate monohydrate)

Cat. No.: HY-W009156 Purity: ≥98.0%
SDS
COA
Handling Instructions Technical Support

Citric acid tripotassium hydrate (Potassium citrate monohydrate) is a natural preservative and food tartness enhancer. Citric acid tripotassium hydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Citric acid tripotassium hydrate cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid tripotassium hydrate is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.

For research use only. We do not sell to patients.

Citric acid tripotassium hydrate Chemical Structure

Citric acid tripotassium hydrate Chemical Structure

CAS No. : 6100-05-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
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500 mg In-stock
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Based on 5 publication(s) in Google Scholar

Other Forms of Citric acid tripotassium hydrate:

Citric acid tripotassium hydrate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Citric acid tripotassium hydrate (Potassium citrate monohydrate) is a natural preservative and food tartness enhancer. Citric acid tripotassium hydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Citric acid tripotassium hydrate cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid tripotassium hydrate is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries[1][2][3][4].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Citric acid (0-12.5 mM; 24 h) tripotassium hydrate shows antiproliferative activity in a dose dependent manner[3].
Citric acid (12.5 mM; 72 h) tripotassium hydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner[3].
Citric acid (12.5 mM; 48 h) tripotassium hydrate increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Citric acid tripotassium hydrate Related Antibodies

Cell Viability Assay[3]

Cell Line: HaCaT cells
Concentration: 0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time: 24 h
Result: Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis[3]

Cell Line: HaCaT cells
Concentration: 12.5 mM
Incubation Time: 0, 12, 24, 48, 72 h
Result: Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dose-dependent manner.

Western Blot Analysis[3]

Cell Line: HaCaT cells
Concentration: 12.5 mM
Incubation Time: 12, 24, 48, 72 h
Result: Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.
In Vivo

Citric acid (120, 240, and 480 mg/kg; i.p.) tripotassium hydrate significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver[1].
Citric acid (120, 240, and 480 mg/kg; i.p.) tripotassium hydrate induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes[1].
Citric acid (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) tripotassium hydrate causes renal toxicity in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 20 g male Kunming mice[2]
Dosage: 120, 240, 480 mg/kg
Administration: i.p.; weekly for 3 weeks
Result: T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.
Clinical Trial
Molecular Weight

324.41

Formula

C6H7K3O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O[K])CC(C(O[K])=O)(O)CC(O[K])=O.O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 120 mg/mL (369.90 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0825 mL 15.4126 mL 30.8252 mL
5 mM 0.6165 mL 3.0825 mL 6.1650 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (308.25 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (247 KB) HNMR (136 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.0825 mL 15.4126 mL 30.8252 mL 77.0630 mL
5 mM 0.6165 mL 3.0825 mL 6.1650 mL 15.4126 mL
10 mM 0.3083 mL 1.5413 mL 3.0825 mL 7.7063 mL
15 mM 0.2055 mL 1.0275 mL 2.0550 mL 5.1375 mL
20 mM 0.1541 mL 0.7706 mL 1.5413 mL 3.8531 mL
25 mM 0.1233 mL 0.6165 mL 1.2330 mL 3.0825 mL
30 mM 0.1028 mL 0.5138 mL 1.0275 mL 2.5688 mL
40 mM 0.0771 mL 0.3853 mL 0.7706 mL 1.9266 mL
50 mM 0.0617 mL 0.3083 mL 0.6165 mL 1.5413 mL
60 mM 0.0514 mL 0.2569 mL 0.5138 mL 1.2844 mL
80 mM 0.0385 mL 0.1927 mL 0.3853 mL 0.9633 mL
100 mM 0.0308 mL 0.1541 mL 0.3083 mL 0.7706 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Citric acid tripotassium hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Citric acid tripotassium6100-05-6Potassium citrateApoptosisEndogenous MetaboliteBacterialantioxidativeapoptosisrenal toxicityMDA levelsmOxidative damageG2/M phaseS phaseHaCaT cellsInhibitorinhibitorinhibit

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