Cholic acid 7-sulfate (Synonyms: 7-Sulfocholic acid)

Cat. No.: HY-126855 Purity: 99.92%: Data Sheet
SDS

COA
Handling Instructions
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Cholic acid 7-sulfate (7-Sulfocholic acid) is a selective agonist targeting TGR5 (EC₅₀=0.17 μM) and a ligand for MHC class I-related protein (MR1). As a gut-restricted TGR5 agonist, cholic acid 7-sulfate binds to TGR5 on enteroendocrine L cells, induces GLP-1 secretion, and improves glucose tolerance in a TGR5-dependent manner. Cholic acid 7-sulfate also acts as an endogenous ligand for MR1, promoting the survival of mucosal-associated invariant T cells MAIT and the expression of homeostatic gene signatures, affecting MAIT cell development and function. Cholic acid 7-sulfate is mainly used in the research of diabetes and MAIT cell-related immune regulation.

For research use only. We do not sell to patients.

Cholic acid 7-sulfate Chemical Structure

CAS No. : 60320-05-0

Size	Stock	Quantity
500 μg	In-stock	Estimated Time of Arrival: December 31
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Other Forms of Cholic acid 7-sulfate:

Cholic acid 7-sulfate-d₄ Get quote

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MHC I

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

TGR^[1]

In Vitro

Cholic acid 7-sulfate activates human TGR5 in a dose-dependent manner and induces GLP-1 secretion from NCI-H716 cells (EC₅₀=0.17 μM), and this effect can be inhibited by TGR5 siRNA knockdown^[1].
Cholic acid 7-sulfate (1 mM; 6 d) promotes MAIT cell survival and induces CXCR4 and IL7R expression when stimulated with human PBMCs, without causing cell proliferation or CD69 upregulation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	PBMCs
Concentration:	1 mM
Incubation Time:	6 days
Result:	Promotes MAIT cell survival.

In Vivo

Cholic acid 7-sulfate (1 mg; enteral administration; single dose; 15 min) increases circulating GLP-1 levels, decreases blood glucose, and increases insulin in diet-induced obese (DIO) mice, while inducing colonic Tgr5 gene expression^[1]. Cholic acid 7-sulfate (100 mg/kg; oral gavage; single dose; 5 h) improves glucose tolerance and increases circulating GLP-1 in DIO mice, and the glucose clearance effect is dependent on TGR5^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

488.63

Formula

C₂₄H₄₀O₈S

CAS No.

60320-05-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])[C@H](OS(=O)(O)=O)C[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])C[C@H](O)[C@]12C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (204.65 mM)

DMF : 1 mg/mL (2.05 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0465 mL	10.2327 mL	20.4654 mL
5 mM	0.4093 mL	2.0465 mL	4.0931 mL
10 mM	0.2047 mL	1.0233 mL	2.0465 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (255 KB) HNMR (203 KB) RP-HPLC (247 KB) MS (80 KB)

Handling Instructions (2659 KB)

References

[1]. Chaudhari SN, et, al. Bariatric surgery reveals a gut-restricted TGR5 agonist with anti-diabetic effects. Nat Chem Biol. 2021 Jan;17(1):20-29. [Content Brief]

[2]. Ito E, et al. Sulfated bile acid is a host-derived ligand for MAIT cells. Sci Immunol. 2024 Jan 26;9(91):eade6924. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF / H₂O	1 mM	2.0465 mL	10.2327 mL	20.4654 mL	51.1635 mL
H₂O	5 mM	0.4093 mL	2.0465 mL	4.0931 mL	10.2327 mL
	10 mM	0.2047 mL	1.0233 mL	2.0465 mL	5.1163 mL
	15 mM	0.1364 mL	0.6822 mL	1.3644 mL	3.4109 mL
	20 mM	0.1023 mL	0.5116 mL	1.0233 mL	2.5582 mL
	25 mM	0.0819 mL	0.4093 mL	0.8186 mL	2.0465 mL
	30 mM	0.0682 mL	0.3411 mL	0.6822 mL	1.7054 mL
	40 mM	0.0512 mL	0.2558 mL	0.5116 mL	1.2791 mL
	50 mM	0.0409 mL	0.2047 mL	0.4093 mL	1.0233 mL
	60 mM	0.0341 mL	0.1705 mL	0.3411 mL	0.8527 mL
	80 mM	0.0256 mL	0.1279 mL	0.2558 mL	0.6395 mL
	100 mM	0.0205 mL	0.1023 mL	0.2047 mL	0.5116 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cholic acid 7-sulfate Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cholic acid 7-sulfate60320-05-07-Sulfocholic acidG protein-coupled Bile Acid Receptor 1MHCG-protein coupled receptor 19GPCR19TGR5GPBAR1Major Histocompatibility ComplexTgr5GLP-1Inhibitorinhibitorinhibit

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