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  3. Biochemical Assay Reagents
  4. Chlorophosphonazo III

Chlorophosphonazo III is a sensitive reagent for both calcium and magnesium. It is possible to determine calcium alone at pH 2.2 and calcium and magnesium at pH 7.0. Chlorophosphonazo III can also be used for the spectrophotometric determination of titanium, zirconium, thorium, and scandium, quinquevalent actinide elements, and protactinium.

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Chlorophosphonazo III

Chlorophosphonazo III Chemical Structure

CAS No. : 1914-99-4

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Description

Chlorophosphonazo III is a sensitive reagent for both calcium and magnesium. It is possible to determine calcium alone at pH 2.2 and calcium and magnesium at pH 7.0. Chlorophosphonazo III can also be used for the spectrophotometric determination of titanium, zirconium, thorium, and scandium, quinquevalent actinide elements, and protactinium[1].

Molecular Weight

757.36

Formula

C22H16Cl2N4O14P2S2
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=P(O)(C1=CC(Cl)=CC=C1/N=N/C2=C(C3=C(C=C2S(=O)(O)=O)C=C(S(=O)(O)=O)C(/N=N/C4=CC=C(C=C4P(O)(O)=O)Cl)=C3O)O)O
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Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 35 mg/mL (46.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3204 mL 6.6019 mL 13.2038 mL
5 mM 0.2641 mL 1.3204 mL 2.6408 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3204 mL 6.6019 mL 13.2038 mL 33.0094 mL
5 mM 0.2641 mL 1.3204 mL 2.6408 mL 6.6019 mL
10 mM 0.1320 mL 0.6602 mL 1.3204 mL 3.3009 mL
15 mM 0.0880 mL 0.4401 mL 0.8803 mL 2.2006 mL
20 mM 0.0660 mL 0.3301 mL 0.6602 mL 1.6505 mL
25 mM 0.0528 mL 0.2641 mL 0.5282 mL 1.3204 mL
30 mM 0.0440 mL 0.2201 mL 0.4401 mL 1.1003 mL
40 mM 0.0330 mL 0.1650 mL 0.3301 mL 0.8252 mL
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Chlorophosphonazo III Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chlorophosphonazo III1914-99-4Biochemical Assay ReagentsInhibitorinhibitorinhibit

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