Chlorhexidine digluconate (20% in water)

Name: Chlorhexidine digluconate (20% in water)
Brand: MedChemExpress
SKU: HY-B0608
Rating: 5.0 (11 reviews)

Cat. No.: HY-B0608 Purity: 99.23%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Chlorhexidine digluconate (20% in water) is a orally active cationic antimicrobial agent that targets microbial cell membranes. Chlorhexidine digluconate (20% in water) binds to cell membrane phospholipids non-specifically, destroys membrane structure and induces leakage of cell contents. Chlorhexidine digluconate (20% in water) has broad-spectrum bactericidal activity against both Gram-positive and Gram-negative bacteria. Chlorhexidine digluconate (20% in water) can interfere with membrane permeability, cause protein precipitation and energy metabolism disorders, such as rapid inhibition of microbial growth and induction of cell death (necrosis or apoptosis).

For research use only. We do not sell to patients.

Chlorhexidine digluconate (20% in water) Chemical Structure

CAS No. : 18472-51-0

Size	Stock	Quantity
Solvent
5 g (222.8 mM * 25 mL in Water)	In-stock	Estimated Time of Arrival: December 31
Solvent
20 g (222.8 mM * 100 mL in Water)	In-stock	Estimated Time of Arrival: December 31

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Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Chlorhexidine digluconate (20% in water):

Chlorhexidine In-stock
Chlorhexidine dihydrochloride Get quote
Chlorhexidine acetate hydrate Get quote
Chlorhexidine diacetate Get quote
Chlorhexidine digluconate (Standard) Get quote

11 Publications Citing Use of MCE Chlorhexidine digluconate (20% in water)

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

1. Cell death assay:
Chlorhexidine digluconate (0.000125%-0.016%; 24 h) induces cell death in L929 fibroblasts, inducing a pattern of cell necrosis and/or apoptosis, as well as cell stress. Furthermore, Chlorhexidine digluconate induces apoptosis at lower concentrations and necrosis at higher concentrations, and increases the expression of heat shock protein 70 (a marker of cell stress). Chlorhexidine digluconate may have an adverse effect on the resolution of apical periodontitis^[1].
2. Cell viability experiment:
Chlorhexidine digluconate (0.0005%-0.5%; 30 min) causes the death of canine embryonic fibroblasts with concentration of ≥0.013%, while allows the cells to survive with concentration of ≤0.006%^[2].
3. Bacterial survival experiment:
Chlorhexidine digluconate (0.0005%-0.5%; 30 min) has a concentration-dependent bactericidal effect on Staphylococcus aureus, and kills Staphylococcus aureus with concentration of ≥0.05%, while allows the bacteria to survive with concentration of ≤0.03%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	Chinese hamster ovary cells
Concentration:	0.00001%, 0.00005%, 0.0001%, 0.0005%
Incubation Time:	1, 2, 4 h
Result:	Demonstrated a cytotoxic effect on CHO cells.

Apoptosis Analysis^[2]

Cell Line:	Chinese hamster ovary cells
Concentration:	0.00005%, 0.0001%, 0.0005%
Incubation Time:	1 h
Result:	After a 1 hour treatment, the amount of cell apoptosis was about 1.62%, 5.51%, and 5.47%, respectively. The amount of cell necrosis was 2.38%, 8.62%, and 22.50%, respectively.

In Vivo

1. Paw injection toxicity test:
Chlorhexidine (0.125%-1.0%; subcutaneous injection; single dose) digluconate causes toxic effects in the plantar space of the hind paw of Balb/c mice. Concentrations ≥0.5% induces coagulative necrosis of the epidermis, dermis and subcutaneous tissues and neutrophil-dominated inflammatory responses, while concentrations ≤0.25% induces moderate inflammation and interstitial edema^[1].
2. Lung inhalation toxicity test:
Chlorhexidine (0.125%-1%; intratracheal instillation; single dose; 8-week observation) digluconate causes collagen fiber deposition and inflammatory cell infiltration in lung tissue in the C57BL/6J mouse model, leading to restrictive ventilation dysfunction. Transcriptome analysis shows that genes related to extracellular matrix deposition and mucus secretion (such as Muc5b and Muc5ac) are upregulated^[3].
3. Genetic toxicity test:
0.12% Chlorhexidine dihydrochloride (0.5 mL; oral, twice a day for 8 days) causes DNA damage in peripheral blood and oral mucosal cells of rats, without causing chromosome breakage or loss in erythrocytes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Imprinting Control Region (ICR) mice at 6 to 8-wk-old (body weight, 25g)^[1]
Dosage:	Chlorhexidine gluconate 0.05% and 0.025%
Administration:	One-time intraperitoneal injection
Result:	With 0.05% lavage had significantly lower incidence of postoperative intra-abdominal abscesses compared with the mice that had saline lavage only.

Clinical Trial

Molecular Weight

897.76

Formula

C₃₄H₅₄Cl₂N₁₀O₁₄

CAS No.

18472-51-0

Appearance

Liquid

Color

Colorless to light yellow

SMILES

N=C(NC(NCCCCCCNC(NC(NC1=CC=C(Cl)C=C1)=N)=N)=N)NC2=CC=C(Cl)C=C2.O[C@H]([C@@H](O)C(O)=O)[C@H](O)[C@H](O)CO.O[C@H]([C@@H](O)C(O)=O)[C@H](O)[C@H](O)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility

In Vitro:

DMSO : ≥ 250 mg/mL (278.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (111.39 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (111.39 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (251 KB) HNMR (315 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Faria G, et al. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured l929 fibroblasts. J Endod. 2007 Jun;33(6):715-22. [Content Brief]

[2]. Sanchez IR, et al. Chlorhexidine diacetate and povidone-iodine cytotoxicity to canine embryonic fibroblasts and Staphylococcus aureus. Vet Surg. 1988;17(4):182-185. [Content Brief]

[3]. Zhang J, et al. Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice. Toxics. 2023 Nov 7;11(11):910. [Content Brief]

[4]. Ribeiro DA, et al. Chlorhexidine induces DNA damage in rat peripheral leukocytes and oral mucosal cells. J Periodontal Res. 2004 Oct;39(5):358-61. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.1139 mL	5.5694 mL	11.1388 mL	27.8471 mL
	5 mM	0.2228 mL	1.1139 mL	2.2278 mL	5.5694 mL
	10 mM	0.1114 mL	0.5569 mL	1.1139 mL	2.7847 mL
	15 mM	0.0743 mL	0.3713 mL	0.7426 mL	1.8565 mL
	20 mM	0.0557 mL	0.2785 mL	0.5569 mL	1.3924 mL
	25 mM	0.0446 mL	0.2228 mL	0.4456 mL	1.1139 mL
	30 mM	0.0371 mL	0.1856 mL	0.3713 mL	0.9282 mL
	40 mM	0.0278 mL	0.1392 mL	0.2785 mL	0.6962 mL
	50 mM	0.0223 mL	0.1114 mL	0.2228 mL	0.5569 mL
	60 mM	0.0186 mL	0.0928 mL	0.1856 mL	0.4641 mL
	80 mM	0.0139 mL	0.0696 mL	0.1392 mL	0.3481 mL
	100 mM	0.0111 mL	0.0557 mL	0.1114 mL	0.2785 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.