2. Autophagy
  3. Autophagy
  4. cGMP-HTL

cGMP-HTL  (Synonyms: cGMP-HaloTag-ligand)

Cat. No.: HY-133869 Purity: 99.71%
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Handling Instructions Technical Support

cGMP-HTL contains a HT-ligand, a linker and the Cys-S-cGMP (autophagy tag). cGMP-HTL increases the K63-linked ubiquitination of mitochondria. AUTAC (autophagy-targeting chimera) is a novel targeted-clearance strategy that contains a degradation tag (guanine derivatives) and a warhead to provide target specificity.

For research use only. We do not sell to patients.

cGMP-HTL

cGMP-HTL Chemical Structure

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Based on 1 publication(s) in Google Scholar

cGMP-HTL Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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Description

cGMP-HTL contains a HT-ligand, a linker and the Cys-S-cGMP (autophagy tag). cGMP-HTL increases the K63-linked ubiquitination of mitochondria. AUTAC (autophagy-targeting chimera) is a novel targeted-clearance strategy that contains a degradation tag (guanine derivatives) and a warhead to provide target specificity[1].

In Vitro

cGMP-HTL (10 μM, 24 h) selective degrades S-guanylated EGFP[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

cGMP-HTL Related Antibodies

Western Blot Analysis[1]

Cell Line: HeLa cells expressing EGFP-HT protein.
Concentration: 10 μM.
Incubation Time: 0-24 h.
Result: Decrease in cGMP-modified EGFP and intact EGFP levels.
Molecular Weight

844.27

Formula

C31H51ClN7O14PS
Appearance

Solid

Color

White to off-white

SMILES

NC(N1)=NC2=C(N=C(SC[C@H](NC(C)=O)C(NCCOCCOCCOCCOCCOCCCCCCCl)=O)N2[C@H]3[C@H](O)[C@H](OP4(O)=O)[C@@H](CO4)O3)C1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (118.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1845 mL 5.9223 mL 11.8446 mL
5 mM 0.2369 mL 1.1845 mL 2.3689 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1845 mL 5.9223 mL 11.8446 mL 29.6114 mL
5 mM 0.2369 mL 1.1845 mL 2.3689 mL 5.9223 mL
10 mM 0.1184 mL 0.5922 mL 1.1845 mL 2.9611 mL
15 mM 0.0790 mL 0.3948 mL 0.7896 mL 1.9741 mL
20 mM 0.0592 mL 0.2961 mL 0.5922 mL 1.4806 mL
25 mM 0.0474 mL 0.2369 mL 0.4738 mL 1.1845 mL
30 mM 0.0395 mL 0.1974 mL 0.3948 mL 0.9870 mL
40 mM 0.0296 mL 0.1481 mL 0.2961 mL 0.7403 mL
50 mM 0.0237 mL 0.1184 mL 0.2369 mL 0.5922 mL
60 mM 0.0197 mL 0.0987 mL 0.1974 mL 0.4935 mL
80 mM 0.0148 mL 0.0740 mL 0.1481 mL 0.3701 mL
100 mM 0.0118 mL 0.0592 mL 0.1184 mL 0.2961 mL
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cGMP-HTL Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

cGMP-HTLcGMP-HaloTag-ligandAutophagyAUTAC,Inhibitorinhibitorinhibit

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