Carbonic anhydrase-IN-2 

Carbonic anhydrase-IN-2 is an orally active carbonic anhydrase inhibitor. Carbonic anhydrase-IN-2 shows antibacterial effect against various vancomycin-resistant Enterococcus (VRE) strains. Carbonic anhydrase-IN-2 can be used for the research of infection, such as E. faecium^[1].

Carbonic anhydrase-IN-2 (Compound 20) (184-3060 nM, 18-20 h) inhibits proliferation of various vancomycin-resistant Enterococcus (VRE) strains including E. faecium NR-31909 and E. faecalis NR-31971, with an MIC₅₀ of 184 nM and an MIC₉₀ of 3060 nM^[1].
Carbonic anhydrase-IN-2 potently inhibits E. faecium α-carbonic anhydrase (Efα-CA) (K_i = 31.6 nM) and γ-carbonic anhydrase (Efγ-CA) (K_i = 62.3 nM)^[1].
Carbonic anhydrase-IN-2 (24 h) shows bacteriostatic effect against E. faecium NR-31909^[1].
Carbonic anhydrase-IN-2 (18-20 h) shows no inhibitory activity against 12 gut commensal strains including Lactobacillus, Bacteroides and Bifidobacterium with MIC >784 μM^[1].
Carbonic anhydrase-IN-2 (0-128 μg/mL, 24 h) shows no obvious cytotoxicity in Vero cells, maintaining cell viability at 95-100%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Carbonic anhydrase-IN-2 (Compound 20) (20 mg/kg, p.o., daily for 3 days, starting 1 h post-infection) significantly improves the survival rate in BALB/c mice infected with E. faecium NR-31909 septicemic peritonitis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

326.39

C₁₂H₁₄N₄O₃S₂

O=S(C1=NN=C(S1)NC(CCC2=CC=C(C=C2)C)=O)(N)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Holly KJ, et al. Hit-to-Lead Optimization of Acetazolamide-Based Bacterial Carbonic Anhydrase Inhibitors with Efficacy In Vivo for Treatment of Vancomycin-Resistant Enterococci Septicemia. J Med Chem. 2025 Sep 11;68(17):18597-18624.  [Content Brief]