2. Stem Cell/Wnt
  3. Wnt
  4. Box5

Box5 is a potent Wnt5a antagonist. Box5 inhibits Wnt5a signaling and inhibits Wnt5a-mediated Ca2+ release. Box5 inhibits cell migration. Box5 has the potential for the research of melanoma.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Box5

Box5 Chemical Structure

CAS No. : 1206604-29-6

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5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Box5:

Box5 Related Antibodies

Top Publications Citing Use of Products

View All Wnt Isoform Specific Products:

View All Isoforms
Wnt1 Wnt3 Wnt5 Wnt7

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Box5 is a potent Wnt5a antagonist. Box5 inhibits Wnt5a signaling and inhibits Wnt5a-mediated Ca2+ release. Box5 inhibits cell migration. Box5 has the potential for the research of melanoma[1].

IC50 & Target

Wnt5A

 

In Vitro

Box5 (100 μM) decreases the expression of rWnt5a (0.1 μg/mL) stimulated p-MARCKS in A2058 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Box5 Related Antibodies

Western Blot Analysis[1]

Cell Line: A2058 cells
Concentration: 100 μM
Incubation Time: Overnight
Result: Decreased the expression of rWnt5a (0.1 μg/mL) stimulated p-MARCKS.
Molecular Weight

766.88

Formula

C30H50N6O13S2
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

{t-Boc}-Met-Asp-Gly-Cys-Glu-Leu
Sequence Shortening

{t-Boc}-MDGCEL
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (130.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3040 mL 6.5199 mL 13.0399 mL
5 mM 0.2608 mL 1.3040 mL 2.6080 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.24%

SDS (252 KB)

COA (247 KB) RP-HPLC (109 KB) MS (230 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3040 mL 6.5199 mL 13.0398 mL 32.5996 mL
5 mM 0.2608 mL 1.3040 mL 2.6080 mL 6.5199 mL
10 mM 0.1304 mL 0.6520 mL 1.3040 mL 3.2600 mL
15 mM 0.0869 mL 0.4347 mL 0.8693 mL 2.1733 mL
20 mM 0.0652 mL 0.3260 mL 0.6520 mL 1.6300 mL
25 mM 0.0522 mL 0.2608 mL 0.5216 mL 1.3040 mL
30 mM 0.0435 mL 0.2173 mL 0.4347 mL 1.0867 mL
40 mM 0.0326 mL 0.1630 mL 0.3260 mL 0.8150 mL
50 mM 0.0261 mL 0.1304 mL 0.2608 mL 0.6520 mL
60 mM 0.0217 mL 0.1087 mL 0.2173 mL 0.5433 mL
80 mM 0.0163 mL 0.0815 mL 0.1630 mL 0.4075 mL
100 mM 0.0130 mL 0.0652 mL 0.1304 mL 0.3260 mL
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Box5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Box51206604-29-6Box 5Box-5WntA2058 cellsrWnt5amelanomaanti-metastaticInhibitorinhibitorinhibit

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