Bodipy TMR-X muscimol

Name: Bodipy TMR-X muscimol
Brand: MedChemExpress
SKU: HY-D1704
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1704 Purity: 99.79%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Bodipy TMR-X muscimol is a Bodipy labeled Muscimol (HY-N2313) (Ex=543 nm, Em=572 nm). Muscimol is a GABAA agonist. Bodipy TMR-X muscimol can be used for imaging the spread of reversible brain inactivations.

For research use only. We do not sell to patients.

Bodipy TMR-X muscimol Chemical Structure

CAS No. : 849464-08-0

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

GABAA receptor^[1]

In Vivo

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[2].
1. Rats are infused with Bodipy TMR-X muscimol as below: dilute the stock solution to 2 μg/μL (in PBS), infused into both hemispheres at a rate of 0.25 μL/min for a single minute, resulting in a final infusion volume of 0.25 μL and a final dose of 0.5 μg per side.
2. The animals are sacrificed by rapid decapitation 15 min after infusion in order to match the spread to what the experimental animals received immediately prior to behavioral testing.
3. The brains are removed and flash frozen in 45°C isopentane, stored at 80°C.
4. Coronal slices are sectioned at 60 μm. The slices are mounted on charged microscope slides and counterstained with DAPI.
5. The stained slices are incubated in a cool, dark room at room temperature for 3 d before being visualized with a confocal microscope.
6. A digital plate from the Paxinos and Watson (2007) rat brain atlas is overlaid on the image to visualize the spread.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

607.46

Formula

C₃₁H₃₆BF₂N₅O₅

CAS No.

849464-08-0

Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

570

Excitation (Ex)

544

SMILES

[F-][B+3]1([N-]2C(C=C(C(C)=C3CCC(NCCCCCC(NCC4=CC(O)=NO4)=O)=O)[N]1=C3C)=CC=C2C5=CC=C(OC)C=C5)[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (164.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6462 mL	8.2310 mL	16.4620 mL
5 mM	0.3292 mL	1.6462 mL	3.2924 mL
10 mM	0.1646 mL	0.8231 mL	1.6462 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (247 KB) HNMR (318 KB) RP-HPLC (231 KB) LCMS (132 KB)

Handling Instructions (2659 KB)

References

[1]. Timothy A Allen, et al. Imaging the spread of reversible brain inactivations using fluorescent muscimol. J Neurosci Methods. 2008 Jun 15;171(1):30-8. [Content Brief]

[2]. Nicholas A Heroux, et al. Differential involvement of the medial prefrontal cortex across variants of contextual fear conditioning. Learn Mem. 2017 Jul 17;24(8):322-330. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6462 mL	8.2310 mL	16.4620 mL	41.1550 mL
	5 mM	0.3292 mL	1.6462 mL	3.2924 mL	8.2310 mL
	10 mM	0.1646 mL	0.8231 mL	1.6462 mL	4.1155 mL
	15 mM	0.1097 mL	0.5487 mL	1.0975 mL	2.7437 mL
	20 mM	0.0823 mL	0.4115 mL	0.8231 mL	2.0577 mL
	25 mM	0.0658 mL	0.3292 mL	0.6585 mL	1.6462 mL
	30 mM	0.0549 mL	0.2744 mL	0.5487 mL	1.3718 mL
	40 mM	0.0412 mL	0.2058 mL	0.4115 mL	1.0289 mL
	50 mM	0.0329 mL	0.1646 mL	0.3292 mL	0.8231 mL
	60 mM	0.0274 mL	0.1372 mL	0.2744 mL	0.6859 mL
	80 mM	0.0206 mL	0.1029 mL	0.2058 mL	0.5144 mL
	100 mM	0.0165 mL	0.0823 mL	0.1646 mL	0.4115 mL