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  3. BODIPY FL alkyne

BODIPY FL alkyne  (Synonyms: BOD FL alkyne)

Cat. No.: HY-114354 Purity: 98.41%
Handling Instructions Technical Support

BODIPY FL alkyne (BOD FL alkyne) is a green-fluorescent dye (Ex=503 nm; Em=512 nm), a bright and photostable fluorophore which emits in fluorescein (FAM) channel. BODIPY FL alkyne can be conjugated with a number of azide-containing molecules by copper-catalyzed Click Chemistry.

For research use only. We do not sell to patients.

BODIPY FL alkyne

BODIPY FL alkyne Chemical Structure

CAS No. : 302795-84-2

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Based on 1 publication(s) in Google Scholar

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Description

BODIPY FL alkyne (BOD FL alkyne) is a green-fluorescent dye (Ex=503 nm; Em=512 nm), a bright and photostable fluorophore which emits in fluorescein (FAM) channel. BODIPY FL alkyne can be conjugated with a number of azide-containing molecules by copper-catalyzed Click Chemistry.

Molecular Weight

329.15

Formula

C17H18BF2N3O

CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

516

Excitation (Ex)

495

SMILES

O=C(NCC#C)CCC1=CC=C(C=C(C(C)=C2)[N]3=C2C)[N-]1[B+3]3([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (151.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0381 mL 15.1906 mL 30.3813 mL
5 mM 0.6076 mL 3.0381 mL 6.0763 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.41%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0381 mL 15.1906 mL 30.3813 mL 75.9532 mL
5 mM 0.6076 mL 3.0381 mL 6.0763 mL 15.1906 mL
10 mM 0.3038 mL 1.5191 mL 3.0381 mL 7.5953 mL
15 mM 0.2025 mL 1.0127 mL 2.0254 mL 5.0635 mL
20 mM 0.1519 mL 0.7595 mL 1.5191 mL 3.7977 mL
25 mM 0.1215 mL 0.6076 mL 1.2153 mL 3.0381 mL
30 mM 0.1013 mL 0.5064 mL 1.0127 mL 2.5318 mL
40 mM 0.0760 mL 0.3798 mL 0.7595 mL 1.8988 mL
50 mM 0.0608 mL 0.3038 mL 0.6076 mL 1.5191 mL
60 mM 0.0506 mL 0.2532 mL 0.5064 mL 1.2659 mL
80 mM 0.0380 mL 0.1899 mL 0.3798 mL 0.9494 mL
100 mM 0.0304 mL 0.1519 mL 0.3038 mL 0.7595 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BODIPY FL alkyne
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