BODIPY 505/515-8-C3-COOH

Name: BODIPY 505/515-8-C3-COOH
Brand: MedChemExpress
SKU: HY-D1581
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1581 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting.

For research use only. We do not sell to patients.

BODIPY 505/515-8-C3-COOH Chemical Structure

CAS No. : 878674-84-1

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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References
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Description

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Fresh N. oceanica cultures are diluted to ~4×10⁶ cells/ml with ASW and kept at 22℃ prior to any treatment. Incubate the cells according to your normal protocol.
2. BODIPY 505/515 is dissolved in DMSO at 4 mg/ml and diluted with DMSO to different working stock concentrations.
3. Cell suspensions are supplemented with the appropriate BODIPY 505/515 working stock to a specific DMSO concentration between 2 and 10% (v/v) with final BODIPY concentrations between 0.8 and 4 μg/ml.
4. Pure DMSO was used for control treatments. 1 ml of fresh culture was diluted to ~4×10⁶ cells/ml with ASW and stained with 6% DMSO and 1.2 μg/ml BODIPY for 15 min (non-stressed cultures) or with 10% DMSO and 1.6 μg/ml BODIPY for 36 min (stressed cultures).
5. Upon addition of the dye, samples were vortexed for 5 s and then incubated in the dark for 15 min before flow cytometric analysis, if not indicated otherwise.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

334.17

Formula

C₁₇H₂₁BF₂N₂O₂

CAS No.

878674-84-1

Appearance

Solid

Color

Orange to red

Emission (Em)

515

Excitation (Ex)

505

SMILES

O=C(CCCC1=C2C(C)=CC(C)=[N]2[B+3]([F-])([N-]3C1=C(C)C=C3C)[F-])O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (299.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9925 mL	14.9624 mL	29.9249 mL
5 mM	0.5985 mL	2.9925 mL	5.9850 mL
10 mM	0.2992 mL	1.4962 mL	2.9925 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (256 KB) HNMR (263 KB) RP-HPLC (244 KB)

Handling Instructions (2659 KB)

References

[1]. ChristianSüdfeld, et al. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515. Algal Research,

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9925 mL	14.9624 mL	29.9249 mL	74.8122 mL
	5 mM	0.5985 mL	2.9925 mL	5.9850 mL	14.9624 mL
	10 mM	0.2992 mL	1.4962 mL	2.9925 mL	7.4812 mL
	15 mM	0.1995 mL	0.9975 mL	1.9950 mL	4.9875 mL
	20 mM	0.1496 mL	0.7481 mL	1.4962 mL	3.7406 mL
	25 mM	0.1197 mL	0.5985 mL	1.1970 mL	2.9925 mL
	30 mM	0.0997 mL	0.4987 mL	0.9975 mL	2.4937 mL
	40 mM	0.0748 mL	0.3741 mL	0.7481 mL	1.8703 mL
	50 mM	0.0598 mL	0.2992 mL	0.5985 mL	1.4962 mL
	60 mM	0.0499 mL	0.2494 mL	0.4987 mL	1.2469 mL
	80 mM	0.0374 mL	0.1870 mL	0.3741 mL	0.9352 mL
	100 mM	0.0299 mL	0.1496 mL	0.2992 mL	0.7481 mL