Bleomycin

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Bleomycin is a glycopeptide antibiotic. Bleomycin has potent antitumour activities against a range of lymphomas, head and neck cancers and germ-cell tumours. Bleomycin can be used for the research of cancer and chemotherapy.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Bleomycin sulfate) that retains the same biological activity.

For research use only. We do not sell to patients.

Bleomycin Chemical Structure

CAS No. : 11056-06-7

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Other In-stock Forms of Bleomycin:

Bleomycin sulfate

Other Forms of Bleomycin:

Bleomycin sulfate In-stock
Bleomycin hydrochloride Get quote

115 Publications Citing Use of MCE Bleomycin

IHC

RT-PCR

: Bleomycin purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187. [Abstract]; Bleomycin sulfate (3 mg/kg; nebulized) significantly upregulates TRPA1 protein levels in mice.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187. [Abstract]; Bleomycin sulfate (3 mg/kg; nebulized) significantly increases TRPA1 mRNA levels in mouse lung tissues.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797. [Abstract]; PCF-EtOH blocked BLM (Bleomycin sulfate)-induced upregulation of β-catenin protein. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797. [Abstract]; PCF-EtOH diminished BLM (Bleomycin sulfate)-induced increase of fibronectin, vimentin and α-SMA. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Mech Ageing Dev. 2024 Jan 11:111902. [Abstract]; miR-129 delays bleomycin-induced cellular senescence. MRC5 cells were transfected with miR-Ctrl or miR-129 and then treated with bleomycin (1 µg/ml) where indicated. Four days after transfection, the p-H2AX, ITPR2, p16, p21 and GAPDH protein levels were quantified by western blot.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Jun 16. [Abstract]; Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in lung sections.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Jun 16. [Abstract]; Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in BALF cells from BLM-treated mice.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Jun 16. [Abstract]; Bleomycin (1 mg/kg; single intratracheal injection). Results for time-course Western blot analysis of Plekhf1 and Arg1 (a macrophage M2 marker) and Col 1α (a fibrotic marker) expression in lung macrophages isolated from BLM-treated mice. Left panel: Representative Western blot images. Right panel: Bar graphs showing the data from three replicates.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]; Bleomycin (BLM) (5 mg/kg; intratracheal instillation) upregulates the mRNA levels of NLRP3, ASC, IL-1β, and IL-18 in BALB/c mice.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]; Bleomycin (BLM) (5 mg/kg; intratracheal instillation) significantly elevated the ratio of phosphorylated p65 to total p65 (p-p65/p65) and reduced the expression level of IκBα, indicating that NF-κB signaling pathway was activated in BLM-induced pulmonary fibrosis.

: Bleomycin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]; Bleomycin (BLM) (5 mg/kg; intratracheal instillation) increases the expression of NLRP3 in BALB/c mice.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Glycopeptide

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	53 μM Compound: Bleomycin	Concentration required to reduce to 50% the number of MCF-7 cells with respect to untreated control after exposure to compound for 24 h Concentration required to reduce to 50% the number of MCF-7 cells with respect to untreated control after exposure to compound for 24 h	[PMID: 12477361]

In Vitro

Bleomycin (0-4 mU/mL; 1 h) induces double-strand break in V79 cells^[2].
Bleomycin (0-1 mU/mL; 1 h) reduces migration of DNA from S-phase cells^[2].
Bleomycin (0-3 μg/mL; 1 h) induces chromatid aberrations in G2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Bleomycin (1 mg and 10 μg; retro-orbitary sinus injection once) affects cells in mitosis, induces apoptosis in tumor models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6 mice with LPB and B16F0 tumours^[4]
Dosage:	1 mg and 10 μg
Administration:	Retro-orbitary sinus injection; 1 mg and 10 μg once
Result:	Decreased in the fraction of cells in mitosis in both tumours, induced cell apoptosis after the electric pulses delivery, inceased the amount of atypical cells in LPB tumours.

Clinical Trial

CAS No.

11056-06-7

SMILES

O=C(C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C)N[C@H](C(N[C@H](C)[C@@H](O)[C@H](C)C(N[C@]([C@H](O)C)([H])C(NCCC2=NC(C3=NC(C(N[R])=O)=CS3)=CS2)=O)=O)=O)[C@H](C4=CN=CN4)O[C@H]5[C@H]([C@H]([C@@H]([C@@H](O5)CO)O)O)O[C@]6([H])[C@H]([C@H]([C@@H]([C@H](O6)CO)O)OC(N)=O)O.NC(NCCCC[R])=N.[R]CCC[S+](C)C.[R=].[Bleomycin A2].[R=].[Bleomycin B2]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Chen J, Stubbe J. Bleomycins: towards better therapeutics. Nat Rev Cancer. 2005;5(2):102-112. [Content Brief]

[2]. Olive PL, et al. Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and 125IdUrd. Int J Radiat Biol. 1993 Oct;64(4):349-58. [Content Brief]

[3]. Allio T, Preston RJ. Increased sensitivity to chromatid aberration induction by bleomycin and neocarzinostatin results from alterations in a DNA damage response pathway. Mutat Res. 2000 Sep 20;453(1):5-15. [Content Brief]

[4]. Mekid H, et al. In vivo evolution of tumour cells after the generation of double-strand DNA breaks. Br J Cancer. 2003 Jun 2;88(11):1763-71. [Content Brief]

Bleomycin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bleomycin11056-06-7AntibioticantibioticV79antitumourcancermigrationdouble-strand breaktumourInhibitorinhibitorinhibit

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