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  3. Biotin-PEG2-alkyne

Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.

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Biotin-PEG2-alkyne

Biotin-PEG2-alkyne Chemical Structure

CAS No. : 2227450-68-0

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Description

Biotin-PEG2-alkyne is a short PEG linker featuring a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azides.

Molecular Weight

369.48

Formula

C17H27N3O4S

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NCCOCCOCC#C)CCCC[C@@H]1SC[C@@H]2NC(N[C@H]12)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (270.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7065 mL 13.5325 mL 27.0651 mL
5 mM 0.5413 mL 2.7065 mL 5.4130 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7065 mL 13.5325 mL 27.0651 mL 67.6627 mL
5 mM 0.5413 mL 2.7065 mL 5.4130 mL 13.5325 mL
10 mM 0.2707 mL 1.3533 mL 2.7065 mL 6.7663 mL
15 mM 0.1804 mL 0.9022 mL 1.8043 mL 4.5108 mL
20 mM 0.1353 mL 0.6766 mL 1.3533 mL 3.3831 mL
25 mM 0.1083 mL 0.5413 mL 1.0826 mL 2.7065 mL
30 mM 0.0902 mL 0.4511 mL 0.9022 mL 2.2554 mL
40 mM 0.0677 mL 0.3383 mL 0.6766 mL 1.6916 mL
50 mM 0.0541 mL 0.2707 mL 0.5413 mL 1.3533 mL
60 mM 0.0451 mL 0.2255 mL 0.4511 mL 1.1277 mL
80 mM 0.0338 mL 0.1692 mL 0.3383 mL 0.8458 mL
100 mM 0.0271 mL 0.1353 mL 0.2707 mL 0.6766 mL
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Biotin-PEG2-alkyne Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Biotin-PEG2-alkyne
Cat. No.:
HY-W615095
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