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  2. Fluorescent Dye
  3. BDP FL maleimide

BDP FL maleimide is a thiol-reactive dye (Ex: 503 nm; Em: 509 nm). BDP FL maleimide can be used for protein labeling, peptide modification, and can replace fluorescein (FAM) for microscopy.

For research use only. We do not sell to patients.

BDP FL maleimide Chemical Structure

BDP FL maleimide Chemical Structure

CAS No. : 773859-49-7

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Based on 1 publication(s) in Google Scholar

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Description

BDP FL maleimide is a thiol-reactive dye (Ex: 503 nm; Em: 509 nm). BDP FL maleimide can be used for protein labeling, peptide modification, and can replace fluorescein (FAM) for microscopy[1].

Molecular Weight

414.21

Formula

C20H21BF2N4O3

CAS No.
Appearance

Solid

Color

Orange to reddish brown

Emission (Em)

509

Excitation (Ex)

503

SMILES

O=C(NCCN(C1=O)C(C=C1)=O)CCC2=CC=C(C=C(C(C)=C3)[N]4=C3C)[N-]2[B+3]4([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (80.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4142 mL 12.0712 mL 24.1423 mL
5 mM 0.4828 mL 2.4142 mL 4.8285 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4142 mL 12.0712 mL 24.1423 mL 60.3559 mL
5 mM 0.4828 mL 2.4142 mL 4.8285 mL 12.0712 mL
10 mM 0.2414 mL 1.2071 mL 2.4142 mL 6.0356 mL
15 mM 0.1609 mL 0.8047 mL 1.6095 mL 4.0237 mL
20 mM 0.1207 mL 0.6036 mL 1.2071 mL 3.0178 mL
25 mM 0.0966 mL 0.4828 mL 0.9657 mL 2.4142 mL
30 mM 0.0805 mL 0.4024 mL 0.8047 mL 2.0119 mL
40 mM 0.0604 mL 0.3018 mL 0.6036 mL 1.5089 mL
50 mM 0.0483 mL 0.2414 mL 0.4828 mL 1.2071 mL
60 mM 0.0402 mL 0.2012 mL 0.4024 mL 1.0059 mL
80 mM 0.0302 mL 0.1509 mL 0.3018 mL 0.7544 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
BDP FL maleimide
Cat. No.:
HY-114350
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