BAP9THP (Synonyms: SD 8339; N-Benzyl-9-(tetrahydro-2h-pyran-2-yl)adenine)

Name: BAP9THP
Brand: MedChemExpress
SKU: HY-119698
Rating: 4.5 (1 reviews)

Cat. No.: HY-119698 Purity: 99.61%: Data Sheet
SDS

COA
Handling Instructions
FAQs
Technical Support

BAP9THP is a synthetic cytokinin derivative and a growth regulator. BAP9THP promotes chlorophyll retention (and senescence delay) in plant tissues exceptionally strongly, and growth of tobacco callus almost as strongly as 6-Benzylaminopurine (BAP). BAP9THP induces adventitious shoot formation ignificantly more strongly than N6-isopentenyladenine or Kinetin.

For research use only. We do not sell to patients.

BAP9THP Chemical Structure

CAS No. : 2312-73-4

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Based on 1 publication(s) in Google Scholar

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	IC₅₀	> 100 μM Compound: 1, PBA	Cytotoxicity against human BJ cells after 72 hrs by MTT assay Cytotoxicity against human BJ cells after 72 hrs by MTT assay	[PMID: 19232496]
K562	IC₅₀	> 100 μM Compound: 1, PBA	Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay	[PMID: 19232496]
MCF7	IC₅₀	> 100 μM Compound: 1, PBA	Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay	[PMID: 19232496]

In Vitro

The cytokinin derivative BAP9THP is an important component of these protocols: culture of isolated apical meristem or growing shoot apices on media containing this compound resulted in signifcant shoot multiplication^[1].
both BAP9THP and BAP9THF are found to delay senescence and induce several growth responses more strongly than BAP^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

309.37

Formula

C₁₇H₁₉N₅O

CAS No.

2312-73-4

Appearance

Solid

Color

White to off-white

SMILES

N1(C=NC2=C(N=CN=C12)NCC3=CC=CC=C3)C4CCCCO4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (323.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2324 mL	16.1619 mL	32.3238 mL
5 mM	0.6465 mL	3.2324 mL	6.4648 mL
10 mM	0.3232 mL	1.6162 mL	3.2324 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (8.08 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.61%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (249 KB) HNMR (263 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Smýkalová, I., et al. The effects of novel synthetic cytokinin derivatives and endogenous cytokinins on the in vitro growth responses of hemp (Cannabis sativa L.) explants. Plant Cell Tiss Organ Cult 139, 381–394 (2019).

[2]. Vylíčilová H, et al. Naturally Occurring and Artificial N9-Cytokinin Conjugates: From Synthesis to Biological Activity and Back. Biomolecules. 2020;10(6):832. Published 2020 May 29. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2324 mL	16.1619 mL	32.3238 mL	80.8094 mL
	5 mM	0.6465 mL	3.2324 mL	6.4648 mL	16.1619 mL
	10 mM	0.3232 mL	1.6162 mL	3.2324 mL	8.0809 mL
	15 mM	0.2155 mL	1.0775 mL	2.1549 mL	5.3873 mL
	20 mM	0.1616 mL	0.8081 mL	1.6162 mL	4.0405 mL
	25 mM	0.1293 mL	0.6465 mL	1.2930 mL	3.2324 mL
	30 mM	0.1077 mL	0.5387 mL	1.0775 mL	2.6936 mL
	40 mM	0.0808 mL	0.4040 mL	0.8081 mL	2.0202 mL
	50 mM	0.0646 mL	0.3232 mL	0.6465 mL	1.6162 mL
	60 mM	0.0539 mL	0.2694 mL	0.5387 mL	1.3468 mL
	80 mM	0.0404 mL	0.2020 mL	0.4040 mL	1.0101 mL
	100 mM	0.0323 mL	0.1616 mL	0.3232 mL	0.8081 mL

BAP9THP Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAP9THP2312-73-4SD 8339 N-Benzyl-9-(tetrahydro-2h-pyran-2-yl)adenineSD8339SD-8339Drug DerivativeDrug derivativecytokininderivativegrowthregulatoradventitiousshootformationInhibitorinhibitorinhibit

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BAP9THP (Synonyms: SD 8339; N-Benzyl-9-(tetrahydro-2h-pyran-2-yl)adenine)

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BAP9THP Related Antibodies

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Complete Stock Solution Preparation Table

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