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  5. AY 9944

AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol.

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AY 9944 Chemical Structure

AY 9944 Chemical Structure

CAS No. : 366-93-8

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of AY 9944:

AY 9944 Related Antibodies

Top Publications Citing Use of Products

    AY 9944 purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2022 Dec 15;209:105497.  [Abstract]

    Pretreatment with AY9944 (15, 30 µM), U18666A, lovastatin and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.

    AY 9944 purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2022 Dec 15;209:105497.  [Abstract]

    AY9944 (30 μМ; 2 h) significantly inhibits the expression of EV-A71 VP1 protein in HCT-8 and RD cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol[1][2][3].

    IC50 & Target

    IC50: 13 nM (DHCR7)1
    In Vitro

    AY 9944 (1 μg/mL; 15 h) affects types of free sterol in keratinocytes[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AY 9944 Related Antibodies

    Cell Viability Assay[2]

    Cell Line: Human foreskin keratinocytes
    Concentration: 1 μg/mL
    Incubation Time: 15 hours
    Result: Inhibited the synthesis of cholesterol and desmosterol, but increased 7-dehydrocholesterol and zymosterol synthesis.
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    AY 9944 (7.5 mg/kg; i.h. every 6 days once from postnatal day 2 to postnatal day 20) reduces brain cholesterol until 400 days after stop treatment[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Long–Evans hooded rats[3].
    Dosage: 7.5 mg/kg
    Administration: Subcutaneous injection; 7.5 mg/kg every 6 days once; from postnatal day 2 to postnatal day 20
    Result: Reduced brain cholesterol and increased 7-dehydrocholesterol in all brain regions of rats till the treatment stopped for 400 days and showed a more severe effect on the change of sterols in plasma and liver of female rats.
    Molecular Weight

    464.30

    Formula

    C22H30Cl4N2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    ClC(C=CC=C1)=C1CNC[C@H](CC2)CC[C@@H]2CNCC3=C(C=CC=C3)Cl.[H]Cl.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 5 mg/mL (10.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 5 mg/mL (10.77 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1538 mL 10.7689 mL 21.5378 mL
    5 mM 0.4308 mL 2.1538 mL 4.3076 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.67%

    SDS (479 KB)

    COA (245 KB) LCMS (89 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.1538 mL 10.7689 mL 21.5378 mL 53.8445 mL
    5 mM 0.4308 mL 2.1538 mL 4.3076 mL 10.7689 mL
    10 mM 0.2154 mL 1.0769 mL 2.1538 mL 5.3844 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    AY 9944 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AY 9944366-93-8AY9944AY-9944Endogenous Metabolitecholesterolreductasebiosynthesis7DHCaccumulationΔ7-Δ8isomerasehypocholesterolemiaInhibitorinhibitorinhibit

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