ATTO 488 

ATTO 488 is a hydrophilic rhodamine-based fluorescent dye with strong absorption and high fluorescence quantum yield. ATTO 488 is suitable for flow cytometry (FACS), fluorescence in situ hybridization (FISH) and most other labeling uses (Ex/Em: 500/520 nm)^[1][2].

1. Preparation of ATTO 488 staining solution:
Prepare a 10 mM stock solution in DMSO and store at −80°C.
2. Labeling procedure:
Add a 2-fold molar excess of ATTO 488 to the purified protein.
Note: Labeling and spectral measurements should be performed quickly under green safe light or in the dark.
3. Incubation procedure:
Incubate for 2 hours at room temperature (RT) or overnight at 4°C with shaking in the dark.
4. Purification:
1) Remove free ATTO 488 using a NAP-5 size exclusion column.
2) Remove traces of free ATTO 488 by ultrafiltration.
5. Characterize the labeled protein by UV/Vis spectroscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

690.05

C₂₅H₂₄ClN₃O₁₄S₂

923585-42-6

Solid

Brown to red

O=C(CCCN(C)C(C1=C(C2=C(C=CC(N)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)(O)=O)C=CC=C1)=O)O.O=Cl(=O)([O-])=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Elkurdi A, et al. Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum. Photochem Photobiol. 2024 May-Jun;100(3):561-572.  [Content Brief]

  [2]. Konrad A, et al. Revealing the radiative and non-radiative relaxation rates of the fluorescent dye Atto488 in a λ/2 Fabry-Pérot-resonator by spectral and time resolved measurements. Nanoscale. 2016 Aug 14;8(30):14541-7.  [Content Brief]