ATP-Red 1 (solution) 

ATP-Red 1 (solution) is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells (Ex/Em = 510/590 nm).

1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Disperse and suspend cells in 100 μL PBS in a 96-well plate at a density of 2×10⁵.
2.2 Add ATP-Red 1 at a final concentration of 5-10 μM.
2.3 Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging).
2.4 Remove ATP-Red 1 working solution.
2.5 Analyze with a fluorescence microscope (Ex/Em = 510/590 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

561.48

C₃₄H₃₆BN₃O₄

1847485-97-5

O=C1N(C2=CC=CC=C2B(O)O)C3(C4=C(OC5=C3C=CC(N(CC)CC)=C5)C=C(N(CC)CC)C=C4)C6=C1C=CC=C6

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wang L, et al. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6  [Content Brief]